Device and method for high throughput screening of crystallization conditions in a vapor diffusion environment

ABSTRACT

A high-density high-throughput microplate and methods for simultaneously screening a plurality of protein crystallization solutions and for producing diffraction quality protein crystals in a vapor-diffusion environment are disclosed. The microplate has defined side-by-side paired chambers of equal size, wherein the side-by-side paired chambers have a maximum volume of about 8 μl, and wherein the paired chambers have a vapor channel, therein providing vapor exchange between the side-by-side paired chambers. The microplate further includes a membrane to seal the surface of the microplate. The microplate is adapted to receive a crystallization solution in one of the side-by-side paired chambers and a protein solution in the other of the side-by-side paired chambers, wherein the protein solution and the crystallization solution interact via a vapor diffusion process, which enables the formation of protein crystals within the chamber that contains the protein solution.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims priority to Application No. 60/983,960 filed on Oct. 31, 2007, the entire contents of which are incorporated by reference herein.

TECHNICAL FIELD

The present invention relates in general to the field of biotechnology and, in particular, to a microplate and methods for simultaneously screening a plurality of protein crystallization solutions and producing diffraction quality protein crystals in a vapor-diffusion environment in a high-density high-throughput format.

BACKGROUND OF THE INVENTION

Various publications, which may include patents, published applications, technical articles and scholarly articles, are cited throughout the specification in parentheses, and full citations of each may be found at the end of the specification. Each of these cited publications is incorporated by reference herein, in its entirety.

Innovative technologies and advancements in experimental techniques have enabled researchers to rapidly increase both the number of newly identified genes and the number of three-dimensional structures of biological macromolecules. There have been significant improvements in the sequential process of gene expression, protein purification, crystallization, and structure determination, but crystallization remains as one of the major bottlenecks in crystal structure determination. To address that issue, a number of different high-throughput protein-crystallization methods have been proposed and a number of automated crystallization systems have been developed (Stevens 2000; Sugahara and Miyano 2002; Sulzenbacher et al. 2002; Watanabe et al. 2002; Hosfield et al. 2003; Hui and Edwards 2003; Stojanoff 2004; Hiraki et al. 2006). For example, the Oryx 6 (Douglas Instruments, Ltd., Berkshire, UK) can set up 96-wells in 12 minutes for sitting-drop vapour diffusion and the Syrrx system can set up 2880 drops for vapour diffusion per hour (Hosfield et al. 2003; Hiraki et al. 2006).

When compared to microbatch and hanging drop methods, sitting-drop vapour-diffusion methods and microplates have advantages for high-throughput crystallization applications. Advantages include easy observation of crystallization drops, easy harvesting of crystals from the drops, and easy handling of the microplates with standard robotics and liquid handling devices (Hiraki et al. 2006). Numerous sitting drop microplates are commercially available at low cost from a number of different vendors, including Hampton Research, Greiner, and Corning. Others, such as Emerald Biostructures Inc., Structural Genomics Inc., and UAB Research Foundation have designed their own microplates or microarrays for custom applications (U.S. Pat. Nos. 6,039,804; 6,656,267; and 7,214,540). Some examples of sitting drop protein crystallography microplates or microarrays are briefly discussed below.

FIG. 1 shows a perspective view (1A) and a cross-sectional side view (1B) of a Cryschem™ Plate from Hampton Research (Hampton Research, Aliso Viejo, Calif.). The Cryschem™ Plate is a 24-well sitting drop microplate that includes an array of twenty-four wells (102), each of which may receive a sample of a protein solution to be assayed. The Hampton Research microplate includes a frame (104) that supports the wells. The frame is rectangular in shape and includes an outer wall (106) and a top planar surface (108) extending between the outer wall and the wells. The wells have circular cross-sections in a plane parallel to the top planar surface. The outer wall that defines the outer periphery of the frame has a bottom edge that extends below the wells. Thus, when the Hampton Research microplate is placed on a support surface, it is supported by the bottom edge (110) with the wells being raised above the support surface to protect them from damage. As illustrated, the outer wall also has a rim (112) to accommodate the skirt of a microplate cover (not shown).

FIG. 1B shows that each well (102) includes outer sidewalls (114), a bottom (116) and a post (118). The post located in the center of the well includes a concaved reservoir (120) in which a protein solution and a reagent solution are placed. A portion of the area in the well around the post receives a reagent solution that has a higher concentration than the protein and reagent solution mixture within the concaved reservoir. The configuration of the well then enables the protein solution and the reagent solution within the concaved reservoir to interact with the reagent solution around the post via a vapor diffusion process, which enables the formation of protein crystals within the concaved reservoir. The typical fill volume for the reagent solution is 500 μl to 1,000 μl, with a total well capacity of 1.5 ml. The maximum drop volume on the post is 40 μl. It should be noted that Hampton Research also has 96-well CrystalClear Strips™ microplates (not shown), in which 50 nanoliters to 4 microliters of protein solution can be dispensed on a shelf on one side of each well and 50 to 100 microliters of crystallization reagent can be placed in the well.

FIG. 2 shows a perspective view (2A), a partial top view (2B) and a cross-sectional side view (2C) of a CrystalQuick™ microplate from Greiner (Greiner Bio-One North America Inc., North Carolina, USA) The Greiner microplate is a 96-well sitting drop microplate where each well (202) may receive up to three samples of protein solutions to be studied. As seen from the perspective view, the Greiner microplate includes a frame (204) that supports the wells. The frame, which is rectangular in shape, includes an outer wall (206) that defines the periphery of the frame and a top planar surface (208) extending between the outer wall and the wells. The wells as shown have rectangular cross-sections in a plane parallel to the top planar surface.

FIG. 2B and FIG. 2C show that each well (202) includes a relatively large reservoir (214) and three relatively small reservoirs (216). Each small reservoir includes a flat bottom (218) on which there can be deposited a protein solution and a reagent solution. The large reservoir located next to the small reservoirs typically receives a reagent solution that has a higher concentration than the reagent solutions within the small reservoirs. The configuration of the well then enables the protein solution and the reagent solution within each of the small reservoirs to interact with the reagent solution within the large reservoir via a vapor diffusion process. This enables the formation of protein crystals within each of the small reservoirs.

FIG. 3 shows a perspective view (3A), a cut-away partial perspective view (3B), and a cross-sectional side view (3C) of a Corning microplate described in U.S. Pat. No. 6,913,732. As illustrated, the microplate is a 96-well high-throughput crystallography microplate that includes an array of ninety-six functional wells (302), each of which are able to receive a sample of a protein solution. The microplate includes a frame (304) that supports the wells. The frame, which is rectangular in shape, includes an outer wall (306) and a top planar surface (308) extending between the outer wall and the wells. As illustrated, the outer wall defines the outer periphery of the frame, which has a bottom edge (310) that extends below the wells. When the microplate is placed on a support surface, it is supported by the bottom edge with the wells raised above the support surface. The outer wall also has a rim to accommodate the skirt of a microplate cover (not shown).

FIG. 3B and FIG. 3C show that each functional well (302) is composed of two overlapping circular wells (302 a and 302 b), both of which are located in a plane parallel to the top planar surface (308). In particular, the first overlapping well has a relatively small concaved reservoir (314) capable of receiving a protein solution and a reagent solution and the second overlapping well has a relatively large reservoir (316) capable of receiving a reagent solution that has a higher concentration than the reagent solution deposited in the first well. After depositing protein solutions and reagent solutions in the wells, the openings of the wells can be covered by a seal such as an adhesive seal or a heat seal to prevent excessive evaporation of the solutions. As a result of the configuration and placement of the first and second overlapping wells, the protein solution and the reagent solution can interact via a vapor diffusion process, which enables the formation of protein crystals within the first well containing the protein solution.

FIG. 4 shows a perspective view (4A), a partial top view (4B), and a cross-sectional side view (4C) of a second microplate design described in U.S. Pat. No. 6,913,732. The microplate shown in FIG. 4 has 96 functional wells in which the first part of the well (402 a) and the second part of the well (402 b) are adjacent to one another and not overlapping as in the wells of the microplate shown in FIG. 3.

FIG. 5 shows a perspective view (5A), a partial top view (5B), and a cross-sectional side view (5C) of a third microplate design described in U.S. Pat. No. 6,913,732. The microplate shown in FIG. 5 has 48 functional wells composed of a first well (502 a) and the second well (502 b) connected to one another by a channel (504). The first well (502 a) includes a relatively small reservoir and the second well (502 b) includes a relatively large reservoir.

In U.S. Pat. No. 7,214,540, there is disclosed a method of screening protein crystal growth conditions with microchambers having a volume from about 0.001 nl to about 250 nl. Also disclosed is a method that employs a microarray with a plurality of wells or reservoirs as shown in FIG. 6. The microarray (10) includes two wells (12, 14) connected by a microchannel (16) that connects the protein solution well (12) and the precipitate solution well (14). It is further disclosed that the wells are sufficient for holding or retaining a desired volume of from about 0.001 nl to about 500 nl, preferably from about 0.01 nl to about 20 nl. Protein crystal growth in the different chambers is monitored by high resolution or other optical means, which automatically detects crystal growth or by manual inspection using high-resolution microscopy or electron microscopy. It is disclosed that if desirable crystal growth is observed in a sample, the protein crystal growth conditions of the sample can be reproduced on a macro scale to produce a protein crystal for further analysis. The very small volumes of the screening methods disclosed do not support growth of large diffraction quality crystals during the screen.

The microplate of the present invention has advantages over other available crystallography microplates. The microplate of the present invention is in a high-density 1536-well format with 768 functional wells, thus allowing for a truly high-density high-throughput screen using a sitting-drop vapor-diffusion method. The standard 1536-well format allows for facile robotic handling of the microplate and compatibility with a wide range of liquid handling systems. Furthermore, using wells of equal size with bottoms aligned in the same plane at the bottom of the wells allows for facile imaging with an inverted light microscope while at the same time allowing manipulation and harvesting of crystals from above. In a preferred embodiment, in which the bottoms of the wells are flat, microscopic images of the wells can be very rapidly screened because the bottoms of the wells are in a single focal plane. It should also be noted that the decreased reservoir to droplet ratio volumes of the high-density high-throughput format should lead to faster equilibration rates and more rapid protein nucleation and crystal growth compared to using other available crystallography microplates (Santarsiero et al. 2002).

The microplate and methods of the present invention also have an advantage over the microarray and methods described in U.S. Pat. No. 7,214,540. By using the microplate of the present invention with 8 μl maximum volumes it is possible to use protein solution volumes of about 1 μl or volumes as much as 2 μl, thus the method of the present invention allows for growth of diffraction quality crystals during a high-density high-throughput screen. The crystals obtained directly from the screen are suitable for analysis by x-ray, thus eliminating the need to reproduce the crystals on a macro scale to produce a protein crystal suitable to be analyzed.

SUMMARY OF THE INVENTION

The present invention includes a microplate and methods for simultaneously screening a plurality of protein crystallization solutions and producing diffraction quality protein crystals in a vapor-diffusion environment in a high-density high-throughput format.

According to a first aspect of the present invention, there is provided a microplate, comprising a frame including a plurality of wells with defined side-by-side paired chambers of equal size, wherein the side-by-side paired chambers have a maximum volume of about 8 μl, wherein the paired side-by-side chambers have a vapor channel providing vapor exchange between the side-by-side paired chambers.

According to a second aspect of the present invention, there is provided a microplate comprising a frame having a footprint that can be easily handled by a robotic handling system.

According to a third aspect of the present invention, there is provided a microplate, wherein the bottoms of the side-by-side paired chambers are aligned in the same plane.

According to a fourth aspect of the present invention, there is provided a microplate, wherein the bottoms of the side-by-side paired chambers are flat, conical, or concave.

According to a fifth aspect of the present invention, there is provided a microplate, wherein the vapor channel has a predetermined depth and width to allow for a predetermined quantity of a first and second crystallization solution to optimally equilibrate.

According to a sixth aspect of the present invention, there is provided a microplate, wherein the vapor channel is formed by a predetermined opening in a portion of a wall between the side-by-side paired chambers and a transparent adhesive membrane that is positioned over the side-by-side paired chambers.

According to a seventh aspect of the present invention, there is provided a microplate, wherein each well is positioned on said frame such that a liquid handling system can automatically deposit a formulated crystallization solution into one of the side-by-side paired chambers and can automatically deposit a protein solution into the other side-by-side paired chamber.

According to an eighth aspect of the present invention, there is provided a microplate, wherein the high-density high-throughput sitting-drop vapor diffusion protein crystallography microplate has 768 functional wells.

According to a ninth aspect of the present invention, there is provided a microplate, wherein each well is positioned on said frame such that a liquid handling system can automatically deposit the formulated crystallization solution into one of the side-by-side paired chambers and can automatically deposit a protein solution into the other side-by-side paired chamber.

According to a tenth aspect of the present invention, there is provided a method wherein a liquid handling system can automatically deposit a formulated crystallization solution into one of the side-by-side paired chambers of a microplate of the present invention and can automatically deposit a protein solution into the other side-by-side paired chamber of a microplate of the present invention, and wherein the protein solution in one side-by-side paired chamber and the crystallization solution within the second side-by-side paired chamber interact via a vapor diffusion process which enables the formation of protein crystals within the chamber containing the protein solution.

According to an eleventh aspect of the present invention, there is provided a method, wherein the formulated crystallization solutions are selected from the solutions shown in Table 2.

According to a twelfth aspect of the present invention, there is provided a method, wherein the amount of formulated crystallization solution deposited is about 6 μl and the amount of protein solution deposited is about 1 μl.

According to a thirteenth aspect of the present invention, there is provided a method, wherein the amount of formulated crystallization solution deposited is in the range of about 4 μl to about 8 μl and the amount of protein solution deposited is in the range of greater than 0.5 μl to about 2 μl.

BRIEF DESCRIPTION OF THE FIGURES

A preferred embodiment of the present invention will now be described, by way of an example only, with reference to the accompanying drawings wherein:

FIG. 1 shows a perspective view (1A) and a cross-sectional side view (1B) of a Cryschem™ Plate from Hampton Research Inc.

FIG. 2 shows a perspective view (2A), a partial top view (2B) and a cross-sectional side view (2C) of a CrystalQuick™ microplate by Greiner Bio-One North America Inc.

FIG. 3 shows a perspective view (3A), a cut-away partial perspective view (3B), and a cross-sectional side view (3C) of a first microplate disclosed in U.S. Pat. No. 6,913,732.

FIG. 4 shows a perspective view (4A), a partial top view (4B), and a cross-sectional side view (4C) of a second microplate disclosed in U.S. Pat. No. 6,913,732.

FIG. 5 shows a perspective view (5A), a partial top view (5B), and a cross-sectional side view (5C) of a third microplate disclosed in U.S. Pat. No. 6,913,732.

FIG. 6 shows a microarray disclosed in U.S. Pat. No. 7,214,540.

FIG. 7 shows a top view (7A) of a modified 1536-well transparent polystyrene assay plate having 768 functional wells, with column 1 and every odd column following designated for crystallization solutions (W) and column 2 and every even column following designated for protein droplets (P). When sealed with a transparent adhesive membrane, the shorter milled wall creates a vapor channel connecting the two side-by-side paired chambers, W and P, thus forming a single environment for crystallization (7B).

FIG. 8 shows 4 functional wells of the crystallography microplate of the present invention. 8A is a side view through the center of four functional wells with column 1 and every odd column following designated for crystallization solutions (W) and with column 2 and every even column following designated for protein droplets (P). 8B shows the side view of 8A with a 90 degree rotation. 8C shows a top view of 4 functional wells of the high-density high-throughput 768 functional well microplate of the present invention with 6 μl of crystallization solution in W and 1 μl of protein solution in P.

FIG. 9 shows images and the associated narrow scoring guidelines used to score each crystallization experiment. Scores from 1 through 10 are critical markers identifying a protein's threshold compared with each solution component. A rating of 10 initially gets grouped with protein leads until it is determined to be salt. Scores from 11 through 20 are flagged for optimization experiments to reproduce crystals for further characterization and diffraction analysis.

Table 1: Stock Components for the 1000 Solution Crystallization Screen: Shown is a table of the stock solution reagent set used to generate the 1000 solution crystallization screen. Stock solutions were either prepared at concentrations based on the solubility information provided in the CRC Handbook of Chemistry or purchased from Hampton Research, Inc.

Table 2: Complete List of 1000 Solutions: Shown is a table listing the composition of all of the 1000 solutions used in the high-density high-throughput screen.

DEFINITIONS

Certain terms are used herein which shall have the meanings set forth as follows.

The term “comprising” means “including principally, but not necessarily solely”. Furthermore, variations of the word “comprising”, such as “comprise” and “comprises”, have correspondingly varied meanings.

The following abbreviations are used herein and throughout the specification:

nl: nanoliter;

μl: microliter;

ml: milliliter;

mm: millimeter;

mg/ml: milligram per millimeter;

° C.: degrees Celsius;

DETAILED DESCRIPTION OF THE INVENTION

The present invention will now be further described in greater detail. It is to be understood at the outset, that the figures and examples provided herein are to exemplify and not to limit the invention and its various embodiments.

Reagent Development for High-Throughput Crystallization

Due to the limited amount of crystallization screens commercially available during the development of the high-throughput crystallization method, a diverse sparse-matrix screen of solutions was designed. Based on the generalization that the crystallization success rate for most proteins is equivalent or greater than 2%, Segelke has suggested that a thorough screen for one protein should consist of approximately 288 crystallization solutions (Segelke 2001). Given the low protein and reservoir requirements of the high-density high-throughput method and microplate of the present invention, it was decided to expand the solution screen to decrease the amount of absent parameter space and improve the chances of producing crystals in a single screen. A 1000 solution screen was developed to cover a crystallization parameter space of approximately 4 times the recommended size discussed by Segelke. In a preferred embodiment, diffraction quality crystals are produced directly from a single 1000 solution screen, but the 1000 solution screen was also designed to provide data on the protein's solubility and information for further optimization of conditions if diffraction quality crystals were not produced during the initial screen.

Ideal components were selected to design a unique 1000 solution screen with a maximum likelihood of generating crystals. Information was gathered from optimum solubility screening articles, the NIST/CARB Biological Macromolecule Crystallization Database, PDB (Brookhaven Protein Data Bank) crystallization parameters, the Hofmeister series, and existing crystallization screens from Hampton Research and Emerald Biosystems (Jancarik and Kim 1991; Saridakis and Chayen 2000). The selected chemicals consisted of 50 precipitants, 12 buffers with alternating pH values, 51 additives, and 8 detergents (Table 1). These chemicals were correlated and entered into the CRYStool™ program (Jena Bioscience GmbH, Germany) to randomly generate 1000 unique solutions. The CRYStool™ program was chosen since it had the capability of producing a screen based on random sampling (Segelke 2001). This reagent set was transferred to a spreadsheet and used to calculate stock reagent concentrations. Selected components were manually combined to create each unique crystallization solution comprising the 1000 solution screen listed in Table 2. The complete set of 1000 solutions is a truly diverse set of solutions with a range of pH, buffers, salts, polymers, alcohols, detergents, and other additives. All of the solutions were prepared in 50 ml conical tubes and transferred into Matrix 96-well deep-well storage blocks (Catalogue #4211, Thermo Fisher Scientific, New Hampshire, USA) for storage at 4° C. Solutions in the deep-well blocks have a shelf life of approximately 1 year.

Modified Microplate Design

A microplate and method were needed to quickly set up and use the 1000 solution screen. Although there are alternative methods available, as many as 95% of all crystallization experiments are set up under a vapor diffusion environment. The traditional vapor diffusion method routinely used for more than 20 years utilizes a 24-well deep-well Linbro plate and a suspended 2 μl protein droplet on a glass coverslip. The protein droplet is typically comprised of a 1:1 ratio of protein to crystallization solution and the drop is suspended over 1 ml of crystallization solution. The vapor diffusion method allows the protein droplet to equilibrate with the crystallization solution with water being extracted from the droplet. As the water is extracted during equilibration, the protein and precipitant concentrations slowly increase in the droplet and thus conditions vary over a broad range to promote nucleation and/or crystal growth. Unfortunately the traditional hanging-drop method using 24-well deep-well Linbro plates and a suspended 2 μl protein droplet on a glass coverslip is an extremely laborious and tedious process. In addition, if conventional 24-well Linbro plates were used to conduct the 1000 solution screen, it would have required 42 plates that would have occupied approximately two cubic feet of incubator space, consumed 1 liter of crystallization solutions by using 1 ml of each crystallization solution per well, and taken approximately 16 hours for experimental set up. A 96-well crystallization plate approach would have reduced the number of plates to 11, decreased the total crystallization solution volume to 80 ml by using 80 μl of each crystallization solution per well, and reduced the time to set up the 1000 solution screen to approximately 3 hours.

The present invention provides a microplate and methods to perform sitting-drop vapor diffusion experiments in modified 1536-well Hibase, clear, polystyrene, flat bottom microplates, with 768 functional wells (FIG. 7 and FIG. 8). The method and microplate increased plate storage capacity, reduced the total crystallization solution consumption to slightly less than 7 ml by using only 6 μl per well, and reduced the time to only about 20 minutes to completely set up a 1000 solution screen. In addition, decreased reservoir to droplet ratio volumes were expected to lead to faster equilibration rates and more rapid protein nucleation and crystal growth (Santarsiero et al. 2002). The unmodified 1536-well, Hibase, clear, polystyrene, flat bottom microplates were purchased from Greiner (Greiner America, Inc., Catalogue #782101). The modified microplates were created by milling about ¼ of the height from the top of the wall between two side-by-side wells, thus producing microplates with 768 functional wells consisting of 768 side-by-side paired chambers. After milling, each chamber has a maximum volume of about 8 μl. The shorter milled wall between side-by-side paired chambers becomes a vapor channel when the microplate is sealed with a transparent adhesive membrane. (FIG. 7 and FIG. 8).

Starting from the left side of the microplate, column 1 and every odd column following are designated for well solutions (W) (FIG. 7 and FIG. 8). Column 2 and every even column following are designated for protein droplets (P) (FIG. 7 and FIG. 8). When sealed with a transparent adhesive membrane, the shorter milled wall creates a vapor channel connecting the two side-by-side paired chambers, W and P, thus forming a single environment for crystallization. For example, one experiment would include a first selection from the 1000 solutions in W₁ and a protein droplet in P₂. A second experiment would include a second selection from the 1000 solutions in W₃ and a protein droplet in P₄. A third experiment would include a third selection from the 1000 solutions in W₅ and a protein droplet in P₆. Each protein droplet is a 1:1 ratio of a stock protein solution and one of the 1000 crystallization solutions that is made by pipetting about 0.5 μl of stock protein solution and 0.5 μl of one of the 1000 crystallization solutions into each protein well. The crystallization solution used in a 1:1 ratio in each protein droplet well (P) is the same as the corresponding crystallization solution used in each side-by-side paired crystallization solution well (W). This procedure continues over the entire modified microplate to set up a complete microplate of 768 crystallization experiments.

Utilization of the Modified Microplate

The 1000 crystallization solutions are transferred from Matrix 96-well deep-well storage blocks (Catalogue #4211, Thermo Fisher Scientific, New Hampshire, USA) using a Gilson C250 robot (Gilson, Inc., Middleton, Wis., USA) into three 384-well daughter plates (Greiner America, Inc., Catalogue #781201). Each daughter plate is made to contain 80 μl per well of one of the 1000 crystallization solutions. Each daughter plate can accommodate a high-throughput screening cycle of 12 proteins before re-dispensation is necessary. The daughter plates are used to dispense the crystallization solutions into the screening microplates. Two modified 1536-well modified microplates with 768 functional wells are required to run a full screen of 1000 solutions. A first microplate is made to contain 768 experiments in 768 functional wells. A second microplate is made to contain the remaining 232 experiments in 232 functional wells with an additional 536 functional wells for expansion of the screen in the future if more solutions are desired.

To add crystallization solutions and protein solutions to the high-density high-throughput 768 functional well screening microplates, a highly reproducible crystallization routine was developed using the VPrep® automated liquid handling system with a fixed 384 syringe head (Velocity 11, Inc., California, USA). In a typical high-density high-throughput screen, the (W) well receives 6 μl of one of the 1000 crystallization solutions from a 384-well daughter plate and the (P) well receives 0.5 μl of stock protein solution and 0.5 μl of one of the 1000 crystallization solutions for a final volume 1 μl. The crystallization solution used in a 1:1 ratio in each protein droplet well (P) is the same as the corresponding crystallization solution used in each side-by-side paired crystallization solution well (W). After setting up the screening microplate, each well solution (W) has a protein droplet (P) adjacent to it at essentially half the concentration of the crystallization solution (FIG. 7 and FIG. 8). The microplate is then sealed with a transparent adhesive membrane and centrifuged at 2500 rpm for 5 minutes to ensure the protein droplet is at the bottom of the protein well. The plates are then stored at either 4° C. or 22° C. until queued for image analysis. Once sealed with the transparent adhesive membrane, which forms the vapor channel from the milled wall between the 768 paired chambers, each protein droplet equilibrates with each well solution until the protein solution reaches the same concentration as the well solution. The process of equilibration promotes nucleation by permitting the protein to be concentrated toward a supersaturated state.

Visualization & Image Analysis

In order to increase both the throughput and precision necessary to evaluate experiments in the high-density high-throughput 768 functional well microplates, an automated Nikon M3 inverted microscope, Phase 3 Imaging XY stage, and an Evolution MP 5.1 Mega-pixel CCD color camera were assembled to capture and record images. The primary focus was to identify crystals for harvesting and analysis by x-ray diffraction or to identify crystallization leads for data analysis and further optimization to enhance crystal quality. Every captured image, 100 KB per frame, is time date stamped and binned in appropriate folders to create a unique figure array for visualization. It takes approximately 1½ hours to image a complete 1000-well experimental set.

Each set of 1000 images uses approximately 100 MB of disk space and is stored in an internal database to be accessed for comparative examination. The Crystal Evaluator browser, designed in-house, is used to load a set of images and visualize each image. Internal control settings include zoom in/out and light intensity filters to assist with accurate scoring. The scoring process is currently done manually, but can be easily adapted into an automated process once image recognition software becomes further automated. Each image is manually scored against an ordinal 20 number ratings schema to define the visual characteristics of the protein crystallization droplet (FIG. 9). The narrow interpretation of each rating assists with the correlation of how each solution component affects protein behaviour. Any droplet having a rating ≧10 is flagged as an initial lead and subsequently is queued for reproducibility and protein validation studies. The ratings are also converted into a binary format of 0 and 1. Any result observed from 1 to 10 is recorded as 0 while results from 11 to 20 are recorded as 1. While results tend to be subjective from observer to observer, the ratings list was generated to specifically define the majority of observations typically observed in a crystallization experiment from the worst to the best. Ratings from 11 through 20 are most important since they identify solutions that produce protein crystals. All results, including negative ones, are recorded in a database to include both ordinal and binary tables and provide data to study trends in protein crystallization from solution to solution. The quality of the leads dictates the path taken for further characterization. Crystals large enough for x-ray studies are harvested directly from the high-density high-throughput 768 functional well microplates, placed into a preformulated cryo-protectant, frozen at −173° C., and screened for protein diffraction. If crystals are too small to x-ray, they are either stained with a Coomassie solution to observe absorption, crushed to determine if protein, or used as a seed stock in crystal regeneration. Optimization experiments are conducted on leads identified with diffraction ≧8 Å. Historical methods to generate improved crystals suitable for structural studies include experiments with variable pH and precipitant concentrations, additive screening, buffer/precipitant substitutions, and seeding.

Results

The 1000 solution set and the high-density high-throughput 768 functional well microplate format and method were initially tested using a 15 mg/ml lysozyme stock solution. The test produced a 17.5% hit rate by identifying 175 unique solutions as leads for crystallizing lysozyme. The hits ranged from crystal showers to crystals larger than 0.5 mm. Crystals, ranging from 0.05 mm to greater than 0.5 mm, comprised 14% of the 1000 solutions, with 2% larger than 0.25 mm. The results confirmed that the 1000 solution set and the high-density high-throughput 768 functional well microplate format and method were suitable for generating protein crystals in a screen and for identifying leads for further optimization and crystal generation.

The 1000 solution set and the high-density high-throughput 768 functional well microplate format and method have become invaluable for the process of rapidly screening proteins to identify leads and produce crystals suitable for structure based drug design. Over the past three years, the process has identified 684 leads resulting in the structure determination of 33 proteins or inhibitor complexes from 13 of the 46 therapeutic targets investigated. Surface response data on proteins from all therapeutic areas against each of the 1000 solutions is currently being collected to build a repository for the calculation and prediction of optimal crystallization conditions for unknown proteins.

TABLE 1 PRECIPITANTS BUFFERS pH ADDITIVES DETERGENTS 2 Ethoxyethanol Bis-Tris Propane 4.5 1,4-Dithio-DL-Threitol C12E9 Ammonium Acetate Bis-Tris Propane 5.5 1,4 Butanediol Cymal-3 Ammonium Bromide Bis-Tris Propane 6.5 1,4 Dioxane Glucopyranoside Ammonium Citrate Bis-Tris Propane 7.5 1,6 Hexanediol Glycerol Ammonium Nitrate Bis-Tris Propane 8.5 2,2,2-Trifluoroethanol LDAO Ammonium Phosphate CAPSO 8.5 Acetonitrile Maltoside Ammonium Sulfate CAPSO 9.5 Ammonium Sulfate Triton X-100 Cadmium Sulfate Monohydrate Gomori's succinate 4.5 ATP disodium salt Zwittergent Calcium Acetate Dihydrate Gomori's succinate 5.5 Barium Chloride Calcium Chloride Gomori's succinate 6.5 Benzamidine HCl Dioxane Hepes 6.5 Betaine Monohydrate Ethanol Hepes 7.5 Cadmium Chloride Ethylene Glycol Hepes 8.5 Calcium Chloride Ferric Chloride Hexahydrate Mopso 6.5 Cesium Chloride Glycerol Mopso 7.5 Cobaltus Chloride Isobutanol Na-Acetate 4.5 Cupric Chloride Isopropanol Na-Acetate 5.5 D (+) Glucose Jeffamine M-600 Na-Cacodylate 4.5 Dextran Sulfate Lithium Chloride Na-Cacodylate 5.5 DMSO Lithium Sulfate Monohydrate Na-Cacodylate 6.5 EDTA Magnesium Chloride Na-Cacodylate 7.5 Ethanol Magnesium Sulfate Na-Citrate 4.5 Ethyl Acetate Methanol Na-Citrate 5.5 Ethylene Glycol MPD Na-Citrate 6.5 Glycerol Anhydrous Nickel Chloride Hexahydrate Na—K-Phosphate 5.5 Glycine PEG 10K Na—K-Phosphate 6.5 Glycyl-Glycyl-Glycine PEG 1500 Na—K-Phosphate 7.5 Guanidine HCl PEG 200 Na—K-Phosphate 8.5 Isopropanol PEG 300 Na-Succinate 4.5 Jeffamine M-600 PEG 400 Na-Succinate 5.5 Lithium Chloride PEG 4K Na-Succinate 6.5 Magnesium Chloride PEG 600 Na-Succinate 7.5 Manganese Chloride PEG 6K TRIS-HCl 6.5 MPD PEG 8K TRIS-HCl 7.5 NAD PEG DME 250 TRIS-HCl 8.5 PEG 200 PEG DME 2K TRIS-Maleate 4.5 PEG 400 PEG MME 550 TRIS-Maleate 5.5 Phenol PEG MME 5K TRIS-Maleate 6.5 Potassium Chloride Polyethyleneimine sec-butanol Potassium Chloride Sodium Chloride Potassium Phosphate Sodium Fluoride Potassium Sodium Tartrate Sodium Iodide Sec-Butanol Sodium Thiocyanate Sodium Acetate Spermidine Sodium Chloride Strontium Chloride Sodium Formate Taurine Sodium Phosphate Trimethylamine HCl Sodium Sulfate Urea Tri-Sodium Citrate Dihydrate Xylitol Zinc Sulfate Hexahydrate Yttrium Chloride Zinc Acetate

TABLE 2 Precip. Final Additive Final Detergent final # Final pH Precipitant Conc. vol #1 50 Buffer Buffer Final Conc. Additive Conc. Detergent Conc. 1 4.57 PEG 6K 23 23.0% 23 Na-Acetate pH 4.5 50 mM Acetonitrile 2.32% 2 7.36 PEG 600 43.2 18.0% 18 Hepes pH 8.5 100 mM Magnesium Chloride 0.204 M 3 5.3 Sec-Butanol 4 4.0% 2 Bis-Tris Propane pH 9.5 100 mM Sodium Fluoride 0.3184 M 4 5.46 Isopropanol 20 20.0% 10 Na-Acetate pH 5.5 50 mM NAD 0.01 M Cymal-3 0.44% 5 6.78 PEG 600 24 24.0% 24 Na-Succinate pH 6.5 100 mM 6 6.98 2 Ethoxyethanol 9.4 9.4% 4.7 TRIS-Maleate pH 6.5 50 mM 1,6 Hexanediol 0.92 M 7 6.16 PEG 600 28.1 28.1% 28.1 Hepes pH 6.5 100 mM Ammonium Sulfate 0.154 M 8 5.91 PEG DME 250 9.2 9.2% 4.6 Hepes pH 6.5 100 mM Cesium Chloride 0.028 M 9 8.75 Jeffamine M-600 9.8 9.8% 4.9 Hepes pH 6.5 100 mM 10 7.94 Glycerol 8.6 8.6% 4.3 TRIS-HCl pH 8.5 100 mM 11 5.93 PEG 1500 26.2 26.2% 26.2 Na-Malonate pH 4.0 100 mM Jeffamine M-600 4.9% 12 7.89 PEG 600 22.1 22.1% 22.1 Hepes pH 8.5 100 mM Sodium Chloride 0.32 M 13 5.84 PEG DME 250 5.8 5.8% 2.9 Mopso pH 6.5 100 mM Lithium Chloride 0.418 M 14 7.88 Isobutanol 5.9 5.9% 2.95 Bis-Tris Propane pH 6.5 100 mM Spermidine 0.02 M 15 6.19 PEG 4K 24.1 24.1% 24.1 Na—K-Phosphate pH 5.5 100 mM Sodium Iodide 0.082 M 16 8.63 Isobutanol 10 10.0% 5 Bis-Tris Propane pH 8.5 100 mM 17 6.04 PEG 200 53.5 53.5% 26.75 Na-Citrate pH 5.5 50 mM Cesium Chloride 0.146 M 18 7.02 PEG MME 550 33.5 33.5% 16.75 Hepes pH 7.5 100 mM Sodium Fluoride 0.156 M 19 5.38 Ethanol 33 33.0% 16.5 TRIS-Maleate pH 4.5 50 mM Glucopyranoside 0.82% 20 5.82 PEG 300 27 27.0% 13.5 Bis-Tris Propane pH 8.5 100 mM Acetonitrile 0.8% 21 4.23 PEG MME 2K 26.4 26.4% 26.4 Na-Cacodylate pH 4.5 100 mM Sodium Chloride 0.29 M 22 4.19 Isobutanol 4.2 4.2% 2.1 Na-Cacodylate pH 4.5 100 mM Cesium Chloride 0.084 M 23 5.67 PEG 10K 14 14.0% 14 Na—K-Phosphate pH 5.5 100 mM Sodium Chloride 0.1 M 24 6.96 PEG 6K 5.2 5.2% 5.2 TRIS-HCl pH 7.5 100 mM Acetonitrile 2.96% 25 7.52 PEG DME 2K 13.2 13.2% 13.2 TRIS-HCl pH 8.5 100 mM Cobaltus Chloride 0.0061 M 26 5.22 Glycerol 54.8 54.8% 27.4 Na-Acetate pH 5.5 50 mM NAD 0.014 M 27 7.76 PEG MME 5K 23.8 23.8% 23.8 Na-Malonate pH 4.0 100 mM Acetonitrile 1.92% 28 6.86 PEG 10K 24.5 24.5% 24.5 TRIS-Maleate pH 6.5 50 mM Ammonium Sulfate 0.189 M 29 8 PEG 6K 25.1 25.1% 25.1 Na—K-Phosphate pH 8.5 100 mM Sodium Thiocyanate 0.192 M 30 5.89 PEG 300 50.1 50.1% 25.05 Hepes pH 6.5 100 mM Sodium Chloride 0.295 M 31 8.25 PEG DME 2K 7 7.0% 7 Bis-Tris Propane pH 7.5 100 mM Spermidine 0.012 M Triton X-100 0.44% 32 6.1 Ethylene Glycol 58.1 58.1% 29.05 Na-Citrate pH 5.5 50 mM Jeffamine M-600 1.15% 33 4.76 PEG DME 2K 26.6 26.6% 26.6 Na-Cacodylate pH 5.5 100 mM Barium Chloride 0.058 M 34 5.08 PEG MME 2K 21 21.0% 21 Na-Citrate pH 4.5 50 mM Lithium Chloride 0.278 M 35 6.12 PEG 4K 22.8 22.8% 22.8 Na-Cacodylate pH 4.5 100 mM Spermidine 0.014 M Glucopyranoside 0.96% 36 5.62 PEG 4K 22.8 22.8% 22.8 Hepes pH 6.5 100 mM 37 8.95 PEG 600 9.4 9.4% 9.4 CAPSO pH 8.5 50 mM 38 7.2 Ethanol 39.6 39.6% 19.8 TRIS-HCl pH 7.5 100 mM Sodium Iodide 0.372 M Cymal-3 0.84% 39 10.57 PEG MME 5K 23.7 23.7% 23.7 Na-Citrate pH 4.5 50 mM Jeffamine M-600 2.2% 40 6.89 Ethylene Glycol 15 15.0% 7.5 Na-Citrate pH 6.5 50 mM Sodium Fluoride 0.2064 M Glycerol   2% 41 4.63 PEG 300 41.8 41.8% 20.9 Na-Citrate pH 4.5 50 mM Lithium Chloride 0.216 M Cymal-3   1% 42 8.12 Ethylene Glycol 5.8 5.8% 2.9 Na—K-Phosphate pH 7.5 100 mM 43 10.65 PEG 400 13.9 13.9% 6.95 Na-Citrate pH 6.5 50 mM Spermidine 0.012 M 44 4.08 PEG DME 250 5.4 5.4% 2.7 Bis-Tris Propane pH 9.5 100 mM Cymal-3 0.24% 45 7 Glycerol 8.4 8.4% 4.2 Na—K-Phosphate pH 6.5 100 mM Cesium Chloride 0.086 M 46 6.2 PEG 400 20 20.0% 10 Na-Succinate pH 6.5 100 mM Cesium Chloride 0.196 M 47 8.49 Ethylene Glycol 31.4 31.4% 15.7 Na—K-Phosphate pH 8.5 100 mM Acetonitrile 1.68% 48 4.47 Sec-Butanol 10 10.0% 5 Na-Succinate pH 4.5 100 mM Acetonitrile 0.8% Triton X-100  0.2% 49 10.31 Jeffamine M-600 40 40.0% 20 CAPSO pH 8.5 50 mM 50 4.35 PEG MME 550 19.8 19.8% 9.9 Na-Malonate pH 4.0 100 mM Glycerol  2.4% 51 5.82 Mopso pH 6.5 100 mM Zinc Acetate 0.012 M 52 5.73 PEG 8K 12.2 12.2% 12.2 Mopso pH 7.5 100 mM Cobaltus Chloride 0.064 M 53 10 Jeffamine M-600 18 18.0% 9 Na-Citrate pH 5.5 25 mM NAD 0.006 M LDAO 0.21% 54 5.36 PEG 10K 12.1 12.1% 12.1 Na-Citrate pH 5.5 50 mM Cesium Chloride 0.216 M 55 4.11 PEG MME 5K 38.3 38.3% 38.3 Na-Malonate pH 4.0 100 mM 56 5.98 Methanol 41.6 41.6% 20.8 Na-Citrate pH 5.5 50 mM C12E9  0.2% 57 6.85 Methanol 30.2 30.2% 15.1 TRIS-Maleate pH 5.5 50 mM 58 6.25 Isopropanol 43.6 43.6% 21.8 Na-Citrate pH 5.5 50 mM 59 6.82 PEG 600 37.2 37.2% 37.2 Mopso pH 6.5 100 mM 60 4.63 Isopropanol 32 32.0% 15.8 Bis-Tris Propane pH 9.5 100 mM Ethanol  2.8% 61 3.06 2 Ethoxyethanol 31.4 31.4% 15.7 TRIS-HCl pH 6.5 100 mM ATP disodium salt 0.018 M C12E9  0.2% 62 10.72 PEG 10K 6.45 6.5% 6.45 0 mM Jeffamine M-600 3.25% 63 7.78 MPD 50 50.0% 25 TRIS-Maleate pH 6.5 50 mM 64 6.13 PEG MME 2K 32.1 32.1% 32.1 Hepes pH 6.5 100 mM 65 6.79 PEG 600 44 44.0% 44 Na-Succinate pH 7.5 100 mM 66 6.49 Isobutanol 3.8 3.8% 1.9 Mopso pH 6.5 100 mM 1,6 Hexanediol 1.04 M 67 3.89 2 Ethoxyethanol 27.5 27.5% 13.75 Hepes pH 6.5 100 mM NAD 0.011 M 68 8.66 PEG 10K 21.7 21.7% 21.7 CAPSO pH 8.5 50 mM Sodium Iodide 0.242 M Glycerol  3.4% 69 10.54 Polyethyleneimine 3 3.0% 3 Na—K-Phosphate pH 8.5 100 mM Sodium Thiocyanate 0.076 M 70 3.33 PEG MME 5K 17.1 17.1% 17.1 0 mM Zinc Acetate 0.134 M 71 6.16 PEG 300 37.4 37.4% 18.7 Na-Cacodylate pH 6.5 100 mM Barium Chloride 0.057 M C12E9  0.2% 72 5.52 PEG 400 31.5 31.5% 15.75 Na-Succinate pH 6.5 100 mM Magnesium Chloride 0.15 M 73 3.71 PEG 4K 19.4 19.4% 19.4 Na-Acetate pH 4.5 50 mM Cupric Chloride 0.051 M 74 9.53 Polyethyleneimine 3.5 3.5% 3.5 Na-Citrate pH 5.5 50 mM Cesium Chloride 0.18 M LDAO 0.11% 75 5.26 Isobutanol 4.4 4.4% 2.2 Na-Cacodylate pH 5.5 100 mM 1,6 Hexanediol 1.88 M 76 4.67 Methanol 45.6 45.6% 22.8 Na-Malonate pH 4.0 100 mM 77 4.28 PEG 300 56.6 56.6% 28.3 TRIS-Maleate pH 4.5 50 mM Zinc Acetate 0.05 M 78 7.75 PEG DME 250 20 20.0% 10 CAPSO pH 8.5 50 mM Lithium Chloride 0.2 M C12E9  0.2% 79 5.95 PEG 6K 5.3 5.3% 5.3 Na-Cacodylate pH 7.5 100 mM Zinc Acetate 0.05 M 80 4 PEG MME 5K 10.1 10.1% 10.1 Na-Cacodylate pH 4.5 100 mM Calcium Chloride 0.03 M 81 5.41 2 Ethoxyethanol 49.4 49.4% 24.7 TRIS-HCl pH 7.5 100 mM Cobaltus Chloride 0.057 M C12E9  0.2% 82 9.03 Jeffamine M-600 58.8 58.8% 29.4 Bis-Tris Propane pH 9.5 100 mM Ammonium Sulfate 0.2 M 83 5.27 Isopropanol 38.2 38.2% 19.1 Na-Citrate pH 4.5 50 mM Cobaltus Chloride 0.001 M 84 3.54 PEG 8K 26.6 26.6% 26.6 TRIS-HCl pH 6.5 100 mM Cesium Chloride 0.09 M 85 5.77 Methanol 59.6 59.6% 29.8 Na-Cacodylate pH 5.5 100 mM 86 8.54 Isopropanol 7.2 7.2% 3.6 Na—K-Phosphate pH 8.5 100 mM Acetonitrile 2.4% Glycerol  8.4% 87 4.43 PEG 4K 30.8 30.8% 30.8 Na-Acetate pH 4.5 50 mM Sodium Chloride 0.17 M 88 8.85 Methanol 28.6 28.6% 14.3 CAPSO pH 8.5 50 mM 89 4.21 Isobutanol 3.8 3.8% 1.9 0 mM Cupric Chloride 0.038 M 90 7.45 PEG 6K 16.3 16.3% 16.3 Na—K-Phosphate pH 8.5 100 mM 91 5.96 Isopropanol 12 12.0% 6 TRIS-Maleate pH 5.5 50 mM Acetonitrile 0.8% 92 3.37 PEG 10K 13 13.0% 13 0 mM 93 6.63 Dioxane 46.4 46.4% 23.2 Hepes pH 7.5 100 mM ATP disodium salt 0.01 M 94 6.86 PEG 6K 26.9 26.9% 26.9 Na-Cacodylate pH 7.5 100 mM 95 7.42 2 Ethoxyethanol 6.8 6.8% 3.4 Na-Succinate pH 7.5 100 mM DMSO 0.9% 96 7.13 Ammonium 2.29 2.3 M 32.71428571 TRIS-HCl pH 6.5 100 mM Sulfate 97 9.11 PEG DME 250 10 10.0% 5 Na-Cacodylate pH 7.5 100 mM Jeffamine M-600 1% C12E9  0.2% 98 5.53 Ethylene Glycol 4.2 4.2% 2.1 Na-Cacodylate pH 6.5 100 mM Magnesium Chloride 0.162 M 99 6.49 Sec-Butanol 10 10.0% 5 Na-Succinate pH 6.5 100 mM Glucopyranoside 0.98% 100 2.18 PEG DME 2K 11.15 11.2% 11.15 0 mM Zinc Acetate 0.19 M 101 4.38 PEG 1500 19.9 19.9% 19.9 Na-Succinate pH 4.5 100 mM Barium Chloride 0.01 M 102 7.96 Sec-Butanol 10 10.0% 5 CAPSO pH 8.5 100 mM Ammonium Sulfate 0.18 M Glucopyranoside 0.64% 103 4.21 PEG 200 39 39.0% 19.5 Na-Cacodylate pH 4.5 100 mM Lithium Chloride 0.43 M LDAO 0.28% 104 6.63 PEG 200 30 30.0% 15 Na-Succinate pH 7.5 100 mM 105 5.8 PEG 600 8.6 8.6% 8.6 Mopso pH 6.5 100 mM Magnesium Chloride 0.206 M 106 7.21 Ethylene Glycol 21.2 21.2% 10.6 Hepes pH 7.5 100 mM Acetonitrile 3.2% Glycerol  5.2% 107 6.81 PEG 400 48.2 48.2% 24.1 Hepes pH 7.5 100 mM Sodium Thiocyanate 0.172 M 108 7.94 PEG MME 550 19.1 19.1% 9.55 TRIS-HCl pH 8.5 100 mM 1,6 Hexanediol 1.36 M 109 6.96 PEG 6K 11.3 11.3% 11.3 Mopso pH 7.5 100 mM Sodium Iodide 0.204 M 110 8.1 PEG 8K 9.2 9.2% 9.2 TRIS-HCl pH 8.5 100 mM Cesium Chloride 0.1 M 111 7.38 PEG MME 5K 11.9 11.9% 11.9 Bis-Tris Propane pH 7.5 100 mM 112 8.16 PEG 4K 30.6 30.6% 30.6 Bis-Tris Propane pH 8.5 100 mM Calcium Chloride 0.045 M 113 4.19 PEG 600 42.8 41.0% 41 Na-Acetate pH 4.5 50 mM Magnesium Chloride 0.134 M 114 5.52 PEG 8K 7.4 7.4% 7.4 Na-Citrate pH 5.5 50 mM Ethanol 2.4% C12E9  0.2% 115 9.53 PEG MME 2K 22.55 22.6% 22.55 CAPSO pH 8.5 50 mM Jeffamine M-600 4.45% 116 6 PEG MME 550 31.1 31.1% 15.55 Mopso pH 6.5 100 mM Potassium Chloride 0.136 M 117 11.08 Dioxane 2.5 2.5% 1.25 0 mM Spermidine 0.02 M Glucopyranoside 0.46% 118 4.65 Na-Citrate pH 5.5 50 mM Cupric Chloride 0.005 M 119 4.4 PEG 200 25.2 25.2% 12.6 Na-Acetate pH 4.5 50 mM ATP disodium salt 0.019 M 120 6.66 PEG 600 41.4 38.5% 38.5 Mopso pH 6.5 100 mM Sodium Iodide 0.116 M 121 5.39 PEG 10K 26 26.0% 26 Na-Cacodylate pH 6.5 100 mM Magnesium Chloride 0.124 M 122 6.15 PEG MME 2K 11.1 11.1% 11.1 TRIS-Maleate pH 5.5 50 mM Jeffamine M-600 0.9% 123 6 2 Ethoxyethanol 39.6 39.6% 19.8 Na-Citrate pH 5.5 50 mM 124 9.66 Ethanol 11.4 11.4% 5.7 CAPSO pH 9.5 50 mM DMSO 1.5% LDAO  0.1% 125 7.07 Dioxane 2.5 2.5% 1.25 Na-Cacodylate pH 7.5 100 mM DMSO 1.5% 126 5.56 PEG 200 54.2 54.2% 27.1 TRIS-Maleate pH 4.5 50 mM Isopropanol 1.4% 127 6.89 MPD 5.2 5.2% 2.6 Na-Citrate pH 6.5 50 mM Sodium Fluoride 0.1104 M 128 7.18 PEG DME 250 10.4 10.4% 5.2 Na-Citrate pH 5.5 50 mM Maltoside 0.42% 129 6.52 Isopropanol 38.6 38.6% 19.3 Na-Cacodylate pH 6.5 100 mM Sodium Iodide 0.188 M 130 4.31 PEG MME 5K 29.4 29.4% 29.4 Na-Acetate pH 4.5 50 mM Calcium Chloride 0.063 M 131 8.53 PEG MME 2K 39.8 39.8% 39.8 Bis-Tris Propane pH 7.5 100 mM Jeffamine M-600 4.8% 132 4.17 PEG 4K 19.7 19.7% 19.7 0 mM Lithium Chloride 0.122 M 133 7.98 PEG 1500 15.7 15.7% 15.7 Hepes pH 8.5 100 mM 134 8.37 PEG 200 38.8 38.8% 19.4 TRIS-HCl pH 8.5 100 mM Spermidine 0.019 M 135 4.66 Isopropanol 18.8 18.8% 9.4 Na-Succinate pH 4.5 100 mM Jeffamine M-600 0.5% 136 4.47 PEG DME 250 7.4 7.4% 3.7 Na-Succinate pH 4.5 100 mM Ammonium Sulfate 0.112 M 137 6.95 Methanol 19.4 19.4% 9.7 TRIS-HCl pH 6.5 100 mM 138 5.11 Ethylene Glycol 3.6 3.6% 1.8 Na-Cacodylate pH 5.5 100 mM 1,6 Hexanediol 0.08 M 139 7.57 MPD 26.4 26.4% 13.2 Na-Citrate pH 6.5 25 mM Lithium Chloride 0.07 M Glycerol  2.4% 140 6.02 Sec-Butanol 4 4.0% 2 0 mM Ethanol 2.6% 141 4.86 PEG DME 250 28 28.0% 14 TRIS-Maleate pH 4.5 50 mM Cesium Chloride 0.024 M 142 7.52 PEG 8K 35.1 35.1% 35.1 TRIS-Maleate pH 6.5 50 mM Jeffamine M-600 1.2% 143 6.23 PEG 200 42 42.0% 21 Mopso pH 7.5 100 mM Cobaltus Chloride 0.01 M 144 5.76 PEG 4K 39.2 39.2% 39.2 Na-Acetate pH 5.5 50 mM Sodium Thiocyanate 0.136 M 145 8.03 PEG 4K 15.7 15.7% 15.7 Hepes pH 8.5 100 mM 146 4.94 PEG 10K 27 27.0% 27 Na-Acetate pH 5.5 50 mM Magnesium Chloride 0.104 M Triton X-100 0.05% 147 10.45 Jeffamine M-600 15 15.0% 7.5 Na—K-Phosphate pH 6.5 100 mM 148 6.15 PEG MME 5K 36.9 36.9% 36.9 Hepes pH 6.5 100 mM 1,6 Hexanediol 0.6 M 149 7.57 PEG MME 550 23.5 23.5% 11.75 TRIS-HCl pH 8.5 100 mM 150 3.08 PEG 6K 9 9.0% 9 0 mM Cupric Chloride 0.01 M 151 6.86 2 Ethoxyethanol 6.4 6.4% 3.2 TRIS-Maleate pH 6.5 50 mM Ethanol 0.2% 152 5.6 PEG 6K 13.4 13.4% 13.4 Na-Citrate pH 5.5 50 mM 1,6 Hexanediol 0.99 M 153 10.23 Polyethyleneimine 2.5 2.5% 2.5 Na—K-Phosphate pH 7.5 100 mM Sodium Chloride 0.28 M 154 10.44 Methanol 54 54.0% 27 Na-Succinate pH 6.5 100 mM Spermidine 0.015 M 155 7.02 Polyethyleneimine 1.5 1.5% 1.5 Na-Acetate pH 4.5 50 mM Cesium Chloride 0.17 M 156 3.93 Sec-Butanol 10 10.0% 5 Na-Malonate pH 4.0 100 mM Calcium Chloride 0.086 M Maltoside  0.4% 157 7.18 Dioxane 2.5 2.5% 1.25 Mopso pH 6.5 100 mM Sodium Chloride 0.35 M 158 9.74 Jeffamine M-600 20 20.0% 10 Na-Cacodylate pH 4.5 100 mM Maltoside  0.2% 159 6.88 PEG DME 250 25.6 25.6% 12.8 Hepes pH 7.5 100 mM Potassium Chloride 0.122 M 160 4.78 Glycerol 37.8 37.8% 18.9 0 mM Ethanol 2.3% 161 7.84 PEG 4K 23.2 23.2% 23.2 Na-Malonate pH 6.0 100 mM Sodium Thiocyanate 0.144 M Cymal-3  0.7% 162 6.05 PEG 8K 6 6.0% 6 Mopso pH 6.5 100 mM EDTA 0.014 M 163 5.97 PEG 4K 8.5 8.5% 8.5 Mopso pH 6.5 100 mM Sodium Chloride 0.16 M C12E9  0.2% 164 8.98 PEG 200 45.8 45.8% 22.9 CAPSO pH 9.5 50 mM 165 7.43 Jeffamine M-600 0.5 0.5% 2.1 Hepes pH 7.5 100 mM 166 3.45 PEG DME 250 18.5 18.5% 9.25 0 mM 1,6 Hexanediol 1.86 M 167 6.86 PEG DME 250 10 10.0% 5 Hepes pH 7.5 100 mM ATP disodium salt 0.011 M LDAO 0.46% 168 7.22 PEG 10K 6.6 6.6% 6.6 Na—K-Phosphate pH 6.5 100 mM Ethanol 3.8% 169 4.81 Methanol 29.2 29.2% 14.6 Na-Cacodylate pH 4.5 100 mM Cobaltus Chloride 0.053 M LDAO 0.41% 170 9.75 2 Ethoxyethanol 21 21.0% 10.5 CAPSO pH 9.5 50 mM EDTA 0.014 M 171 5.05 2 Ethoxyethanol 43.2 43.2% 21.6 Na-Citrate pH 4.5 50 mM Ammonium Sulfate 0.182 M 172 10.56 Polyethyleneimine 2.3 2.3% 2.3 Na-Succinate pH 6.5 100 mM Sodium Iodide 0.184 M 173 5.22 PEG MME 2K 28.6 28.6% 28.6 Na-Citrate pH 5.5 50 mM Sodium Chloride 0.45 M 174 7.21 Ethanol 40.4 40.4% 20.2 Hepes pH 7.5 100 mM 175 3.28 PEG MME 550 23.7 23.7% 11.85 0 mM Cadmium Chloride 0.008 M 176 6.41 Sec-Butanol 4 4.0% 2 Mopso pH 6.5 100 mM Cesium Chloride 0.158 M 177 9.39 Ethylene Glycol 4.4 4.4% 2.2 CAPSO pH 9.5 50 mM Barium Chloride 0.01 M 178 8.62 PEG 600 27.2 27.2% 27.2 CAPSO pH 9.5 50 mM NAD 0.012 M 179 7.37 PEG 10K 20 20.0% 20 Mopso pH 6.5 100 mM Potassium Chloride 0.05 M LDAO 0.12% 180 6.75 PEG 600 53.6 14.0% 14 Bis-Tris Propane pH 7.5 100 mM Ethanol 4.2% 181 4.93 Isopropanol 40.6 40.6% 20.3 Bis-Tris Propane pH 9.5 100 mM Lithium Chloride 0.26 M 182 4.2 PEG 4K 34.7 34.7% 34.7 Na-Cacodylate pH 4.5 100 mM Acetonitrile 2.16% 183 8.45 Ethylene Glycol 3.8 3.8% 1.9 Bis-Tris Propane pH 8.5 100 mM Acetonitrile 2.72% 184 6.88 Sec-Butanol 20 20.0% 10 0 mM Ethanol 10% 185 8.93 Jeffamine M-600 8 8.0% 4 Na-Acetate pH 4.5 50 mM Ethanol 2% 186 9.74 PEG 300 17.8 17.8% 8.9 CAPSO pH 9.5 50 mM 187 8.4 Sec-Butanol 10 10.0% 5 Bis-Tris Propane pH 8.5 100 mM Sodium Thiocyanate 0.08 M 188 4.64 Ammonium 2 2.0 M 28.57142857 Na-Citrate pH 4.5 50 mM Sulfate 189 7.37 Isopropanol 39 39.0% 19.5 Na-Cacodylate pH 4.5 100 mM Jeffamine M-600 4.7% 190 7.89 PEG 1500 9.8 9.8% 9.8 Mopso pH 7.5 100 mM Potassium Chloride 0.128 M 191 4.36 MPD 37.8 37.8% 18.9 Bis-Tris Propane pH 6.5 100 mM Cobaltus Chloride 0.08 M LDAO 0.11% 192 7.2 PEG MME 2K 30.4 30.4% 30.4 Na-Succinate pH 7.5 100 mM Ammonium Sulfate 0.224 M 193 4.13 Isobutanol 2.8 2.8% 1.4 Na-Cacodylate pH 4.5 100 mM Lithium Chloride 0.174 M 194 7.8 PEG 300 26.4 26.4% 13.2 Na-Malonate pH 4.0 100 mM Cesium Chloride 0.224 M 195 8.32 Methanol 46.4 46.4% 23.2 Bis-Tris Propane pH 8.5 100 mM Magnesium Chloride 0.23 M Cymal-3  0.2% 196 7.95 Ethylene Glycol 5.8 5.8% 2.9 Bis-Tris Propane pH 7.5 100 mM Ammonium Sulfate 0.11 M 197 7.03 Isopropanol 40 40.0% 20 Na-Succinate pH 6.5 100 mM 198 10.16 Jeffamine M-600 26.6 26.6% 13.3 Mopso pH 7.5 100 mM Acetonitrile 3.76% 199 6.43 Isopropanol 44.4 44.4% 22.2 Na-Citrate pH 5.5 50 mM NAD 0.018 M 200 7.84 MPD 42.8 42.8% 21.4 TRIS-HCl pH 8.5 100 mM Calcium Chloride 0.029 M 201 6.68 PEG 400 19.8 19.8% 9.9 Na-Succinate pH 7.5 100 mM Acetonitrile 1.04% 202 4.63 MPD 8.6 8.6% 4.3 TRIS-Maleate pH 4.5 50 mM EDTA 0.011 M 203 7.26 PEG 300 31.8 31.8% 15.9 Na-Citrate pH 5.5 50 mM Sodium Chloride 0.2 M 204 5.59 Ethylene Glycol 23 23.0% 11.5 0 mM Cadmium Chloride 0.03 M 205 5.43 PEG 4K 8.4 8.4% 8.4 Na-Succinate pH 5.5 100 mM 206 10.63 Jeffamine M-600 8.4 8.4% 4.2 Na—K-Phosphate pH 7.5 100 mM Ethanol 3.6% 207 5.86 2 Ethoxyethanol 29.4 29.4% 14.7 Bis-Tris Propane pH 8.5 100 mM DMSO 1.32% Maltoside 0.24% 208 9.66 Methanol 23.4 23.4% 11.7 CAPSO pH 9.5 50 mM DMSO 0.72% LDAO  0.5% 209 9.58 Jeffamine M-600 10 10.0% 5 Na—K-Phosphate pH 6.5 100 mM 210 6.41 PEG 300 27 27.0% 13.5 Na—K-Phosphate pH 5.5 100 mM Ethanol 2% Glycerol  8.2% 211 6.1 PEG DME 250 10.2 10.2% 5.1 Bis-Tris Propane pH 8.5 100 mM Lithium Chloride 0.17 M 212 10.12 PEG 400 10.4 10.4% 5.2 Na—K-Phosphate pH 7.5 100 mM Jeffamine M-600 2.5% 213 7.83 Ammonium 3 3.0 M 42.85714286 TRIS-HCl pH 7.5 100 mM Sulfate 214 7.08 Isopropanol 9.4 9.4% 4.7 TRIS-Maleate pH 6.5 50 mM 1,6 Hexanediol 1.2 M 215 6.17 Na-Cacodylate pH 6.5 100 mM 1,6 Hexanediol 1.68 M Glycerol   8% 216 11.03 Isobutanol 3 3.0% 1.5 0 mM Spermidine 0.017 M 217 5.35 PEG 8K 33.2 33.2% 33.2 Na-Citrate pH 4.5 50 mM 218 7.59 MPD 13.2 13.2% 6.6 Na-Succinate pH 7.5 100 mM Ethanol 2.4% Cymal-3  0.2% 219 3.2 PEG DME 250 10 10.0% 5 0 mM ATP disodium salt 0.006 M 220 5.2 PEG MME 5K 13.3 13.3% 13.3 0 mM Ethanol 0.4% 221 6.17 Isobutanol 4 4.0% 2 Hepes pH 6.5 100 mM 1,6 Hexanediol 1.64 M 222 4.79 PEG MME 5K 18.4 18.4% 18.4 Na-Citrate pH 4.5 50 mM Glucopyranoside  0.2% 223 10.56 MPD 53.6 53.6% 26.8 Na-Succinate pH 7.5 100 mM Jeffamine M-600 4.2% Glycerol  3.4% 224 7.13 Ethylene Glycol 4.2 4.2% 2.1 Hepes pH 6.5 100 mM Spermidine 0.012 M 225 9.2 PEG 200 26.4 26.4% 13.2 Na-Succinate pH 6.5 100 mM Jeffamine M-600 1.2% 226 5.42 PEG MME 550 32.8 32.8% 16.4 Na-Malonate pH 6.0 100 mM 227 6.46 PEG 200 16.8 16.8% 8.4 Na-Cacodylate pH 7.5 100 mM Cupric Chloride 0.01 M 228 6.99 Na-Citrate pH 6.5 50 mM EDTA 0.018 M 229 4.47 PEG 200 27.4 27.4% 13.7 Na-Citrate pH 4.5 50 mM Sodium Chloride 0.26 M 230 6.07 PEG 10K 28.4 28.4% 28.4 Hepes pH 6.5 100 mM Sodium Chloride 0.36 M 231 9.14 2 Ethoxyethanol 17.2 17.2% 8.6 CAPSO pH 9.5 50 mM 232 7.72 Polyethyleneimine 1.7 1.7% 1.7 Na-Cacodylate pH 5.5 100 mM DMSO 1.38% 233 8.68 PEG 4K 26.4 26.4% 26.4 Bis-Tris Propane pH 8.5 100 mM Potassium Chloride 0.19 M 234 9.59 PEG MME 5K 7.7 7.7% 7.7 CAPSO pH 9.5 50 mM Sodium Chloride 0.2 M 235 8.62 PEG 200 6.6 6.6% 3.3 Bis-Tris Propane pH 8.5 100 mM Potassium Chloride 0.07 M 236 4.33 PEG 4K 14.4 14.4% 14.4 CAPSO pH 9.5 50 mM NAD 0.024 M 237 4.45 PEG 200 46.8 46.8% 23.4 TRIS-Maleate pH 4.5 50 mM Lithium Chloride 0.408 M 238 6.67 PEG 400 9.8 9.8% 4.9 Na-Succinate pH 7.5 100 mM Potassium Chloride 0.06 M 239 7.98 PEG 6K 13 13.0% 13 TRIS-HCl PH 8.5 100 mM EDTA 0.022 M C12E9  0.2% 240 6.54 PEG DME 250 10 10.0% 5 TRIS-HCl pH 7.5 100 mM Ammonium Sulfate 0.112 M 241 10.99 Polyethyleneimine 2.8 2.8% 2.8 Na-Citrate pH 4.5 50 mM 242 4.48 Glycerol 13.8 13.8% 6.9 0 mM Zinc Acetate 0.148 M 243 7.43 MPD 54.4 54.4% 27.2 Na-Succinate pH 6.5 100 mM 244 5.77 Ethylene Glycol 10.8 10.8% 5.4 Na-Cacodylate pH 6.5 100 mM ATP disodium salt 0.019 M 245 4.5 PEG 1500 11.6 11.6% 11.6 TRIS-Maleate pH 4.5 50 mM Potassium Chloride 0.054 M 246 3.82 PEG 4K 25.4 25.4% 25.4 Na-Citrate pH 4.5 50 mM Zinc Acetate 0.04 M 247 4.05 PEG 8K 25.4 25.4% 25.4 TRIS-Maleate pH 4.5 50 mM Magnesium Chloride 0.106 M 248 8.29 PEG MME 5K 36.8 36.8% 36.8 Bis-Tris Propane pH 8.5 100 mM Acetonitrile 1.6% 249 5.8 MPD 27 27.0% 13.5 Bis-Tris Propane pH 9.5 100 mM Ammonium Sulfate 0.1 M 250 5.92 PEG MME 550 35 35.0% 17.5 Bis-Tris Propane pH 8.5 100 mM Barium Chloride 0.03 M 251 4.67 PEG 8K 14.2 14.2% 14.2 Na-Citrate pH 4.5 50 mM 252 6 Glycerol 34.4 34.4% 17.2 TRIS-Maleate pH 5.5 50 mM 2,2,2-Trifluoroethanol 4% 253 4.61 Ethylene Glycol 32.8 32.8% 16.4 Na-Acetate pH 4.5 50 mM 254 7.89 PEG MME 2K 19.6 19.6% 19.6 Na—K-Phosphate pH 8.5 100 mM Taurine 0.065 M 255 4.7 PEG DME 250 10 10.0% 5 Na-Citrate pH 4.5 50 mM Dextran Sulfate 1.56% 256 3.89 PEG MME 2K 15 15.0% 15 0 mM PEG 400 2.9% Cymal-3 0.84% 257 5.63 2 Ethoxyethanol 24.8 24.8% 12.4 Na-Succinate pH 5.5 100 mM Trimethylamine HCl 0.082 M 258 8.6 Glycerol 19.4 19.4% 9.7 CAPSO pH 8.5 50 mM Strontium Chloride 0.09 M 259 6.45 Glycerol 29.6 29.6% 14.8 Na-Citrate pH 6.5 50 mM Ethyl Acetate 1.81% 260 4.56 PEG MME 550 12.9 12.9% 6.45 Na-Succinate pH 4.5 100 mM 2,2,2-Trifluoroethanol 1.1% 261 6.63 Sec-Butanol 12 12.0% 6 Na-Succinate pH 6.5 100 mM Glycine 0.108 M 262 3.8 PEG 10K 19.2 19.2% 19.2 TRIS-HCl pH 6.5 100 mM Glycerol Anhydrous 1% 263 6.45 MPD 38.6 38.6% 19.3 Na-Cacodylate pH 6.5 100 mM Manganese Chloride 0.007 M 264 8.35 Methanol 36.4 36.4% 18.2 Bis-Tris Propane pH 8.5 100 mM D (+) Glucose 5% 265 5.75 Isobutanol 9 9.0% 4.5 0 mM sec-butanol 9% Maltoside  0.3% 266 7.15 Isobutanol 5.4 5.4% 2.7 TRIS-HCl pH 7.5 100 mM 2,2,2-Trifluoroethanol 1.1% 267 6.1 PEG MME 550 19.1 19.1% 9.55 Na-Cacodylate pH 6.5 100 mM Xylitol 1% 268 7.81 PEG 400 37.6 37.6% 18.8 Hepes pH 8.5 100 mM Betaine Monohydrate 0.01 M 269 8.75 Polyethyleneimine 3.9 3.9% 3.9 Hepes pH 7.5 100 mM Betaine Monohydrate 0.036 M Triton X-100 0.24% 270 7.03 Dioxane 2.5 2.5% 1.25 TRIS-Maleate pH 6.5 50 mM 1,4 Butanediol 3.8% 271 4.55 Dioxane 2.5 2.5% 1.25 Na-Citrate pH 4.5 50 mM 272 4.49 PEG 4K 12 12.0% 12 TRIS-Maleate pH 5.5 50 mM PEG 400 2% Triton X-100 0.92% 273 5.6 PEG MME 550 24 24.0% 12 TRIS-Maleate pH 5.5 50 mM Glycyl-Glycyl-Glycine 0.015 M 274 10.49 Polyethyleneimine 2.7 2.7% 2.7 0 mM Urea 0.092 M 275 7.72 Glycerol 43.6 43.6% 21.8 Na—K-Phosphate pH 8.5 100 mM Ethylene Glycol 1.2% LDAO 0.42% 276 8.63 PEG 6K 22.6 22.6% 22.6 Na—K-Phosphate pH 8.5 100 mM 2,2,2-Trifluoroethanol 1% 277 7.2 Ethanol 4.2 4.2% 2.1 Na—K-Phosphate pH 6.5 100 mM Sodium Chloride 0.01 M 278 5.81 PEG 200 19 19.0% 9.5 Bis-Tris Propane pH 8.5 100 mM Ethylene Glycol 4% 279 6.32 Sec-Butanol 10 10.0% 5 0 mM Xylitol 3% 280 6.24 PEG 200 56.6 56.6% 28.3 TRIS-HCl pH 7.5 100 mM 1,4-Dithio-DL-Threitol 0.079 M 281 8.3 Isopropanol 20 20.0% 10 Hepes pH 8.5 100 mM 282 5.33 PEG 8K 6.8 6.8% 6.8 Na-Succinate pH 5.5 100 mM Dextran Sulfate 2.58% 283 4.6 PEG DME 250 16.6 16.6% 8.3 Na-Acetate pH 4.5 50 mM MPD 1% Cymal-3  0.1% 284 6.39 PEG 200 22.6 22.6% 11.3 Na-Succinate pH 6.5 100 mM Glycine 0.254 M 285 6.24 PEG 1500 29 29.0% 29 TRIS-Maleate pH 5.5 50 mM sec-butanol 1.3% 286 5.98 PEG 6K 29.7 29.7% 29.7 Hepes pH 6.5 100 mM Guanidine HCl 0.042 M 287 5.63 2 Ethoxyethanol 17.2 17.2% 8.6 Na-Succinate pH 5.5 100 mM 288 3.65 PEG 10K 9.3 9.3% 9.3 TRIS-HCl pH 6.5 100 mM Strontium Chloride 0.011 M C12E9  0.2% 289 7.33 Mopso pH 6.5 100 mM ATP disodium salt 0.01 M 290 7.51 MPD 26.2 26.2% 13.1 Na-Succinate pH 7.5 100 mM Glycyl-Glycyl-Glycine 0.005 M 291 6.92 PEG 1500 33.8 33.8% 33.8 Na-Cacodylate pH 7.5 100 mM D (+) Glucose 1.1% 292 6.41 PEG DME 2K 5.8 5.8% 5.8 0 mM 1,4-Dithio-DL-Threitol 0.079 M 293 6.46 Methanol 18.8 18.8% 9.4 Na-Succinate pH 6.5 100 mM Ethyl Acetate 1.68% 294 3.41 PEG DME 250 26 26.0% 13 0 mM Betaine Monohydrate 0.006 M 295 5.02 Ethylene Glycol 40.8 40.8% 20.4 TRIS-Maleate pH 4.5 50 mM sec-butanol 4.6% 296 7.66 PEG 4K 29.1 29.1% 29.1 Na-Citrate pH 5.5 50 mM 297 7.09 PEG 400 38 38.0% 19 Na-Cacodylate pH 6.5 100 mM 298 6.15 Isopropanol 42 42.0% 21 Hepes pH 6.5 100 mM sec-butanol 1% 299 7.78 PEG 300 49.4 49.4% 23.7 Na-Citrate pH 4.5 50 mM Guanidine HCl 0.02 M Glucopyranoside  0.4% 300 7.76 PEG DME 250 36.2 36.2% 18.1 Hepes pH 8.5 100 mM Guanidine HCl 0.088 M 301 8.99 Isobutanol 5.6 5.6% 2.8 CAPSO pH 8.5 50 mM D (+) Glucose 1.5% 302 6.1 Glycerol 32.6 32.6% 16.3 Na-Cacodylate pH 6.5 100 mM 1,4 Butanediol 1.3% 303 4.63 MPD 21.6 21.6% 10.8 Na-Succinate pH 4.5 100 mM PEG 400 2% 304 7.96 PEG 4K 9 9.0% 9 TRIS-HCl pH 8.5 100 mM Glycerol  5.4% 305 7.59 PEG MME 550 33.9 33.9% 16.95 Na-Malonate pH 6.0 100 mM Glycine 0.036 M 306 4.79 PEG 8K 34 34.0% 34 Na-Cacodylate pH 5.5 100 mM 1,4 Butanediol 2.7% 307 5.01 PEG DME 250 20 20.0% 10 Na-Cacodylate pH 5.5 100 mM Glycine 0.286 M 308 6.23 PEG 8K 23.3 23.3% 23.3 Na-Succinate pH 6.5 100 mM 1,4 Butanediol 0.5% 309 7.88 MPD 47.8 47.8% 23.9 TRIS-HCl pH 7.5 100 mM 310 4.79 Ethanol 36.6 36.6% 18.3 Na-Acetate pH 4.5 50 mM Trimethylamine HCl 0.092 M 311 8.08 PEG 1500 19.5 19.5% 19.5 Hepes pH 8.5 100 mM 1,4-Dithio-DL-Threitol 0.065 M Glucopyranoside 0.36% 312 5.2 PEG 8K 33.8 33.8% 33.8 TRIS-Maleate pH 4.5 50 mM Ethylene Glycol 3.6% 313 7.46 Sec-Butanol 6.6 6.6% 3.3 Bis-Tris Propane pH 7.5 100 mM — Ethylene Glycol 4.5% 314 7.97 PEG DME 250 10 10.0% 5 TRIS-HCl pH 8.5 100 mM 315 8.03 Methanol 19 19.0% 9.5 Na-Citrate pH 4.5 50 mM Glycerol   2% 316 5.99 Isobutanol 4.4 4.4% 2.2 Bis-Tris Propane pH 8.5 100 mM PEG 400 2.6% 317 5.43 PEG 1500 28.1 28.1% 28.1 Na-Malonate pH 7.0 100 mM 2,2,2-Trifluoroethanol 4.3% Glucopyranoside  0.4% 318 3.34 PEG 10K 9.7 9.7% 9.7 0 mM Taurine 0.1 M Triton X-100 0.28% 319 6.96 PEG DME 250 20 20.0% 10 Na-Succinate pH 7.5 100 mM Glycine 0.146 M 320 9.69 PEG 6K 15.2 15.2% 15.2 CAPSO pH 9.5 50 mM PEG 400 2.5% 321 5.45 PEG 300 14 14.0% 7 Na-Succinate pH 5.5 100 mM Guanidine HCl 0.046 M Glucopyranoside  0.4% 322 8.56 PEG 4K 15.5 15.5% 15.5 CAPSO pH 8.5 50 mM 323 7.46 PEG DME 250 44 44.0% 22 TRIS-HCl pH 8.5 100 mM PEG 400 1% 324 7.22 MPD 20 20.0% 10 Hepes pH 7.5 100 mM 325 7.29 PEG 10K 29.7 29.7% 29.7 Na—K-Phosphate pH 8.5 100 mM 326 10.56 Jeffamine M-600 46.8 46.8% 23.4 CAPSO pH 9.5 50 mM Phenol 0.033 M 327 8.1 Sec-Butanol 10 10.0% 5 TRIS-HCl pH 8.5 100 mM Guanidine HCl 0.02 M LDAO 0.42% 328 4.8 PEG 10K 18.7 18.7% 18.7 Na-Cacodylate pH 5.5 100 mM ATP disodium salt 0.019 M 329 5.42 PEG 600 57.4 39.0% 39 Na-Acetate pH 4.5 50 mM Glycyl-Glycyl-Glycine 0.001 M 330 9.72 Jeffamine M-600 6 6.0% 3 Na—K-Phosphate pH 5.5 100 mM Xylitol 4% 331 7.66 PEG 400 22.8 22.8% 11.4 Na—K-Phosphate pH 7.5 100 mM Ethylene Glycol 1% 332 6.17 PEG 8K 10.5 10.5% 10.5 Na-Succinate pH 6.5 100 mM Dextran Sulfate 2.4% 333 8.68 2 Ethoxyethanol 56.4 56.4% 28.2 CAPSO pH 8.5 50 mM D (+) Glucose 1% 334 6.08 Ethylene Glycol 57.8 57.8% 28.9 Hepes pH 6.5 100 mM 2,2,2-Trifluoroethanol 1% 335 3.94 Glycerol 57.2 57.2% 28.6 Na-Cacodylate pH 5.5 100 mM Dextran Sulfate 2.28% 336 5.94 PEG MME 550 38.6 38.6% 38.6 Na-Succinate pH 5.5 100 mM 1,4 Butanediol 3.4% 337 3.7 PEG 4K 31 31.0% 31 Na-Cacodylate pH 4.5 100 mM Strontium Chloride 0.028 M 338 5.41 PEG MME 5K 12.4 12.4% 12.4 Na-Acetate pH 5.5 50 mM 1,4 Butanediol 3.7% 339 4.69 PEG 8K 34.2 34.2% 34.2 0 mM 1,4-Dithio-DL-Threitol 0.088 M 340 5.66 MPD 54.6 54.6% 27.3 Hepes pH 6.5 100 mM Strontium Chloride 0.047 M 341 6.26 Sec-Butanol 10 10.0% 5 Mopso pH 6.5 100 mM Strontium Chloride 0.096 M 342 5.62 PEG MME 2K 24 24.0% 24 Na-Succinate pH 5.5 100 mM 343 4.64 PEG MME 5K 24.8 24.8% 24.8 Na-Succinate pH 4.5 100 mM Urea 0.006 M 344 7.25 TRIS-HCl pH 7.5 100 mM Phenol 0.078 M 345 6.15 PEG DME 250 26.2 26.2% 13.1 TRIS-Maleate pH 5.5 50 mM 1,4-Dithio-DL-Threitol 0.006 M Triton X-100 0.62% 346 7.7 Ethanol 53.8 53.8% 26.9 CAPSO pH 9.5 50 mM Ethyl Acetate 1.38% 347 7.06 Sec-Butanol 10 10.0% 5 TRIS-Maleate pH 6.5 50 mM Ethylene Glycol 1.2% 348 5.6 PEG DME 2K 5 5.0% 5 Na-Citrate pH 5.5 50 mM Taurine 0.077 M 349 5.25 Ethylene Glycol 36 36.0% 17.9 Na-Cacodylate pH 5.5 100 mM Glycerol Anhydrous 2.2% C12E9  0.2% 350 4.75 PEG 6K 18.4 18.4% 18.4 Na-Citrate pH 4.5 50 mM 1,4-Dithio-DL-Threitol 0.056 M 351 5.56 Sec-Butanol 10 10.0% 5 Na-Citrate pH 5.5 50 mM Guanidine HCl 0.06 M 352 5.07 2 Ethoxyethanol 40.2 40.2% 20.1 Na-Succinate pH 4.5 100 mM sec-butanol 2.5% 353 5.68 PEG DME 250 10.6 10.6% 5.3 TRIS-Maleate pH 5.5 50 mM Benzamidine HCl 3.16% 354 5.38 Sec-Butanol 10 10.0% 5 Na-Acetate pH 5.5 50 mM Glycyl-Glycyl-Glycine 0.028 M 355 9.48 Polyethyleneimine 1.4 1.4% 1.4 Hepes pH 8.5 100 mM Ethylene Glycol 2.6% 356 7 PEG 600 49 19.0% 19 Mopso pH 6.5 100 mM Xylitol 4% LDAO 0.17% 357 6.71 PEG 10K 20 20.0% 20 Mopso pH 7.5 100 mM Yttrium Chloride 0.031 M 358 8.83 PEG MME 5K 18.3 18.3% 18.3 CAPSO pH 8.5 50 mM Ethyl Acetate 1% 359 6.58 Isobutanol 4 4.0% 2 Bis-Tris Propane pH 8.5 100 mM Ethyl Acetate 1.53% 360 6.88 PEG 10K 32.7 32.7% 32.7 Na-Citrate pH 6.5 50 mM MPD 1.5% 361 8.35 MPD 26.6 26.6% 13.3 Bis-Tris Propane pH 8.5 100 mM 362 6.86 PEG 1500 24.1 24.1% 24.1 Na-Citrate pH 6.5 50 mM Taurine 0.006 M 363 4.4 Na-Succinate pH 4.5 100 mM Dextran Sulfate 2.52% 364 5.13 PEG 4K 38.7 38.7% 38.7 Na-Cacodylate pH 5.5 100 mM 2,2,2-Trifluoroethanol 2.7% 365 4.89 PEG 300 58.6 58.6% 29.3 0 mM 366 2.95 Glycerol 45.2 45.2% 22.6 0 mM Ethyl Acetate 2.15% 367 5.63 TRIS-Maleate pH 5.5 50 mM Sodium Iodide 0.024 M 368 7.13 Ethanol 10.6 10.6% 5.3 TRIS-HCl pH 7.5 100 mM 2,2,2-Trifluoroethanol 1% C12E9  0.2% 369 5.4 Sec-Butanol 11.2 11.2% 5.6 Na-Acetate pH 5.5 50 mM Phenol 0.052 M 370 2.92 PEG 400 21.8 21.8% 10.9 0 mM Ethyl Acetate 2.86% 371 9.49 Jeffamine M-600 20 20.0% 10 Bis-Tris Propane pH 8.5 100 mM Betaine Monohydrate 0.02 M Triton X-100  0.4% 372 7.7 PEG 400 22 22.0% 11 TRIS-HCl pH 8.5 100 mM 1,4 Dioxane 1.5% Glucopyranoside 0.74% 373 6.86 Isobutanol 2 2.0% 1 Na-Citrate pH 6.5 50 mM Trimethylamine HCl 0.065 M 374 4.64 PEG 300 9.4 9.4% 4.7 Na-Citrate pH 4.5 50 mM D (+) Glucose 1.3% 375 4.6 MPD 26.4 26.4% 13.2 Na-Malonate pH 4.0 100 mM Taurine 0.032 M 376 4.66 PEG 4K 16 16.0% 16 TRIS-Maleate pH 4.5 50 mM Phenol 0.063 M 377 7.73 Ethylene Glycol 49.4 49.4% 24.7 TRIS-HCl pH 8.5 100 mM sec-butanol 3% 378 7.19 PEG DME 250 14.4 14.4% 7.2 Bis-Tris Propane pH 7.5 100 mM Glycine 0.09 M 379 7.09 Glycerol 12.8 12.8% 6.4 Na—K-Phosphate pH 6.5 100 mM ATP disodium salt 0.01 M 380 6.72 2 Ethoxyethanol 19.4 19.4% 9.7 Mopso pH 6.5 100 mM Taurine 0.007 M 381 9.26 PEG DME 250 32.4 32.4% 16.2 CAPSO pH 9.5 50 mM Xylitol 1% 382 6.98 PEG MME 2K 34.4 34.4% 34.4 TRIS-HCl pH 7.5 100 mM Urea 0.037 M Triton X-100  0.7% 383 6.72 PEG 4K 23.2 23.2% 23.2 Mopso pH 7.5 100 mM Guanidine HCl 0.046 M 384 6.19 PEG 200 46 46.0% 23 Mopso pH 6.5 100 mM Benzamidine HCl 1.48% 385 6.33 TRIS-HCl pH 6.5 100 mM ATP disodium salt 0.019 M 386 8.2 Sec-Butanol 12 12.0% 6 Hepes pH 8.5 100 mM D (+) Glucose 4.7% 387 8.7 PEG MME 2K 6.2 6.2% 6.2 CAPSO pH 8.5 50 mM Glycerol Anhydrous 2.6% 388 4.72 Methanol 54.4 54.4% 27.2 Na-Cacodylate pH 4.5 100 mM D (+) Glucose 2.9% 389 4.57 PEG MME 2K 16.4 16.4% 16.4 Na-Acetate pH 4.5 50 mM 390 7.36 PEG 600 12.4 12.4% 12.4 Bis-Tris Propane pH 7.5 100 mM Glycerol Anhydrous 4.4% Cymal-3 0.52% 391 6.98 PEG 200 19 19.0% 9.5 TRIS-HCl pH 7.5 100 mM Cymal-3  0.4% 392 4.99 Ethylene Glycol 23 23.0% 11.5 Na-Cacodylate pH 5.5 100 mM Benzamidine HCl 2.16% 393 9 Jeffamine M-600 20 20.0% 10 Bis-Tris Propane pH 9.5 100 mM 1,4 Butanediol 1% Glycerol  4.8% 394 6.12 Isobutanol 4 0.4% 2 Hepes pH 6.5 100 mM Ethyl Acetate 1.12% 395 2.48 PEG DME 2K 5 5.0% 5 Bis-Tris Propane pH 9.5 100 mM Ethyl Acetate 2.6% 396 5.1 PEG MME 550 19.6 19.6% 9.8 Na-Acetate pH 5.5 50 mM ATP disodium salt 0.006 M Glucopyranoside 0.88% 397 5.48 PEG MME 2K 25 25.0% 25 Na-Acetate pH 5.5 50 mM Glycine 0.288 M 398 6.2 TRIS-HCl pH 6.5 100 mM Yttrium Chloride 0.005 M 399 7.23 Isopropanol 24 24.0% 12 TRIS-Maleate pH 6.5 50 mM Taurine 0.006 M 400 4.4 PEG 6K 9.2 9.2% 9.2 Na-Succinate pH 4.5 100 mM PEG 400 1% 401 7.04 MPD 8.6 8.6% 4.3 Na-Citrate pH 6.5 50 mM 402 9.02 PEG DME 2K 5 5.0% 5 CAPSO pH 8.5 50 mM Xylitol 3% Cymal-3 0.92% 403 5.96 Ethanol 6.6 6.6% 3.3 0 mM Manganese Chloride 0.034 M 404 8.04 Ethylene Glycol 5.4 5.4% 2.7 Na—K-Phosphate pH 7.5 100 mM D (+) Glucose 1% 405 3.9 PEG DME 2K 5 5.0% 5 Na-Cacodylate pH 4.5 100 mM D (+) Glucose 9.5% 406 3.24 PEG DME 2K 7 7.0% 7 0 mM Strontium Chloride 0.06 M 407 6.06 PEG 4K 28.4 28.4% 28.4 Mopso pH 6.5 100 mM 1,4 Butanediol 3.3% 408 7.22 PEG 8K 20.3 20.3% 20.3 Na—K-Phosphate pH 7.5 100 mM 409 4.73 PEG 10K 20 20.0% 20 TRIS-Maleate pH 4.5 50 mM MPD 1% Glycerol  9.2% 410 5.79 PEG 4K 25.3 25.3% 25.3 Bis-Tris Propane pH 7.5 100 mM MPD 3.3% 411 3.66 PEG 300 17.8 17.8% 8.9 TRIS-HCl pH 6.5 100 mM 1,4 Dioxane 1% 412 6.38 Polyethyleneimine 1.5 1.5% 1.5 Na-Acetate pH 4.5 50 mM 1,4 Dioxane 3.4% 413 5.16 MPD 40 40.0% 40 TRIS-Maleate pH 4.5 50 mM 1,4-Dithio-DL-Threitol 0.006 M 414 7.29 PEG MME 5K 12.9 12.9% 12.9 Na-Succinate pH 7.5 100 mM 415 8.83 2 Ethoxyethanol 14 14.0% 7 CAPSO pH 8.5 50 mM 2,2,2-Trifluoroethanol 1.9% 416 7.66 PEG 300 26.6 26.6% 13.3 Na-Citrate pH 5.5 50 mM 2,2,2-Trifluoroethanol 4.5% Glucopyranoside 0.82% 417 5 Methanol 24.8 24.8% 12.4 TRIS-Maleate pH 4.5 50 mM Phenol 0.086 M 418 2.87 PEG 1500 10.6 10.6% 10.6 Na-Cacodylate pH 4.5 100 mM Yttrium Chloride 0.076 M 419 4.58 PEG 6K 26.7 26.7% 26.7 Na-Succinate pH 4.5 100 mM Xylitol 1% 420 11.02 Jeffamine M-600 10 10.0% 5 Na-Citrate pH 6.5 50 mM 1,4-Dithio-DL-Threitol 0.03 M 421 5.83 PEG 300 42.6 42.6% 21.3 Hepes pH 6.5 100 mM ATP disodium salt 0.019 M 422 6.92 Glycerol 31 31.0% 15.5 TRIS-Maleate pH 6.5 50 mM sec-butanol 1.5% C12E9  0.2% 423 9.75 Jeffamine M-600 7 7.0% 3.5 Na-Succinate pH 4.5 100 mM 2,2,2-Trifluoroethanol 1% Glucopyranoside  0.2% 424 7.78 Polyethyleneimine 2.1 2.1% 2.1 Na-Succinate pH 4.5 100 mM Strontium Chloride 0.005 M 425 7.88 PEG 6K 13.7 13.7% 13.7 Na-Malonate pH 4.0 100 mM MPD 1.3% 426 9.53 Jeffamine M-600 6 6.0% 3 Na-Succinate pH 4.5 100 mM PEG 400 0.5% Glucopyranoside 0.68% 427 10.58 Polyethyleneimine 3.7 3.7% 3.7 Na—K-Phosphate pH 7.5 100 mM 428 4.35 Methanol 12 12.0% 6 Na-Malonate pH 4.0 100 mM Trimethylamine HCl 0.065 M 429 9.09 Sec-Butanol 8.8 8.8% 4.4 CAPSO pH 8.5 50 mM Xylitol 3.1% Glucopyranoside 0.38% 430 6.89 PEG 200 24.2 24.2% 12.1 Na-Citrate pH 6.5 50 mM Benzamidine HCl 0.4% Maltoside 0.32% 431 6.1 Bis-Tris Propane pH 7.5 100 mM ATP disodium salt 0.019 M 432 4.56 2 Ethoxyethanol 12 12.0% 6 Na-Acetate pH 4.5 50 mM MPD 1.9% 433 6.03 PEG MME 550 12 12.0% 6 Bis-Tris Propane pH 7.5 100 mM Manganese Chloride 0.043 M 434 5.15 PEG MME 2K 19.7 19.7% 19.7 0 mM Taurine 0.085 M 435 7.26 MPD 33.8 33.8% 16.9 Na-Citrate pH 6.5 50 mM D (+) Glucose 1.9% 436 4.24 PEG MME 2K 10.7 10.7% 10.7 TRIS-HCl pH 7.5 100 mM Ethyl Acetate 1.59% 437 4.1 PEG 4K 35 35.0% 35 Na-Cacodylate pH 4.5 100 mM 2,2,2-Trifluoroethanol 2% Glycerol  6.4% 438 7.1 PEG MME 2K 12.3 12.3% 12.3 Hepes pH 7.5 100 mM Trimethylamine HCl 0.025 M 439 4.43 PEG 8K 22.5 22.5% 22.5 Na-Malonate pH 4.0 100 mM Glycyl-Glycyl-Glycine 0.001 M 440 4.49 Sec-Butanol 5.8 5.8% 2.9 Na-Cacodylate pH 5.5 100 mM Yttrium Chloride 0.005 M 441 5.8 PEG MME 2K 28.3 28.3% 28.3 Na-Citrate pH 5.5 50 mM sec-butanol 1.2% 442 6.12 PEG DME 250 20 20.0% 10 Mopso pH 6.5 100 mM Xylitol 4% 443 7.12 PEG 8K 25.9 25.9% 25.9 Bis-Tris Propane pH 7.5 100 mM 1,4-Dithio-DL-Threitol 0.03 M 444 6.14 PEG MME 5K 10 10.0% 10 Na-Cacodylate pH 6.5 100 mM MPD 1% 445 4.68 Isopropanol 59.4 59.4% 29.7 Na-Cacodylate pH 4.5 100 mM Benzamidine HCl 0.4% Glucopyranoside  0.4% 446 3.75 PEG DME 250 12 12.0% 6 0 mM Phenol 0.048 M Glycerol   2% 447 8.93 Ethanol 32.6 32.6% 16.3 CAPSO pH 8.5 50 mM 1,4 Dioxane 1% 448 10.34 Jeffamine M-600 35 35.0% 17.5 CAPSO pH 8.5 50 mM sec-butanol 2.6% 449 7.22 2 Ethoxyethanol 26.8 26.8% 13.4 Hepes pH 7.5 100 mM 450 4.59 PEG 10K 21.9 21.9% 21.9 Na-Acetate pH 4.5 50 mM 1,4 Butanediol 4.3% 451 5.84 PEG 1500 36.6 36.6% 36.6 CAPSO pH 9.5 50 mM Yttrium Chloride 0.047 M 452 3.35 PEG 1500 22 22.0% 22 0 mM sec-butanol 3% 453 5.56 Ethylene Glycol 31.4 31.4% 15.7 0 mM sec-butanol 4.1% 454 9.98 Polyethyleneimine 3.1 3.1% 3.1 Hepes pH 8.5 100 mM Guanidine HCl 0.07 M 455 8.26 Polyethyleneimine 1 1.0% 1 Bis-Tris Propane pH 7.5 100 mM 456 5.66 PEG 10K 16.5 16.5% 16.5 Na-Cacodylate pH 6.5 100 mM Benzamidine HCl 4.16% 457 3.75 PEG MME 2K 32.1 32.1% 32.1 Na-Cacodylate pH 5.5 100 mM 458 4.78 PEG 600 34.6 34.6% 34.6 Na-Acetate pH 4.5 50 mM Taurine 0.044 M 459 3.46 PEG 600 36.2 36.2% 36.2 0 mM Taurine 0.074 M 460 7.76 PEG 400 15.6 15.6% 7.8 TRIS-HCl pH 8.5 100 mM sec-butanol 4.7% 461 6.65 PEG 200 29.4 29.4% 14.7 Na—K-Phosphate pH 8.5 100 mM Ethyl Acetate 2.27% 462 7.82 MPD 37.4 37.4% 18.7 Na-Citrate pH 6.5 50 mM 1,4-Dithio-DL-Threitol 0.03 M 463 10.82 Jeffamine M-600 10 10.0% 5 Na-Cacodylate pH 7.5 100 mM 464 4.73 Isobutanol 9.2 9.2% 4.6 Na-Citrate pH 4.5 50 mM Glycyl-Glycyl-Glycine 0.005 M 465 8.82 Polyethyleneimine 2.2 2.2% 2.2 Na—K-Phosphate pH 5.5 100 mM Taurine 0.092 M 466 7.42 Isobutanol 4 4.0% 2 Hepes pH 7.5 100 mM 467 5.1 PEG MME 5K 18.6 18.6% 18.6 0 mM Urea 0.01 M 468 7.06 Polyethyleneimine 1.5 1.5% 1.5 Hepes pH 6.5 100 mM Betaine Monohydrate 0.097 M Glycerol  5.6% 469 9.72 MPD 12.6 12.6% 6.3 CAPSO pH 9.5 50 mM 1,4 Dioxane 2.2% 470 3.98 PEG 6K 6.1 6.1% 6.1 TRIS-HCl pH 6.5 100 mM Strontium Chloride 0.086 M 471 3.58 Na-Acetate pH 4.5 50 mM Cupric Chloride 0.066 M 472 5.87 Methanol 15.6 15.6% 7.8 Bis-Tris Propane pH 7.5 100 mM Strontium Chloride 0.02 M 473 8.07 PEG MME 5K 14.5 14.5% 14.5 Na—K-Phosphate pH 7.5 100 mM Guanidine HCl 0.02 M 474 6.71 PEG 300 48.2 48.2% 24.1 TRIS-HCl pH 7.5 100 mM 1,4-Dithio-DL-Threitol 0.017 M Cymal-3 0.62% 475 8.33 Ethylene Glycol 46.4 46.4% 23.2 Na—K-Phosphate pH 7.5 100 mM 1,4 Dioxane 3.1% Triton X-100 0.84% 476 8.86 Ethanol 31.6 31.6% 15.8 CAPSO pH 8.5 50 mM 1,4 Butanediol 4.5% 477 4.85 PEG 4K 10.2 10.2% 10.2 TRIS-HCl pH 6.5 100 mM 478 6.86 PEG DME 2K 5 5.0% 5 Hepes pH 7.5 100 mM ATP disodium salt 0.011 M 479 6.23 MPD 5.2 5.2% 2.6 TRIS-HCl pH 6.5 100 mM Phenol 0.079 M 480 6.19 Mopso pH 6.5 100 mM Strontium Chloride 0.062 M 481 9.29 Methanol 26.2 26.2% 13.1 Na—K-Phosphate pH 8.5 100 mM sec-butanol 2.9% 482 7.03 Sec-Butanol 5 5.0% 2.5 Na-Citrate pH 6.5 50 mM 483 6.58 Ethanol 20.6 20.6% 10.3 Na-Succinate pH 6.5 100 mM Manganese Chloride 0.026 M 484 7.17 Dioxane 2.5 2.5% 1.25 Hepes pH 7.5 100 mM D (+) Glucose 4.3% 485 7.23 PEG DME 250 6.6 6.6% 3.3 Na—K-Phosphate pH 6.5 100 mM Urea 0.065 M 486 7.99 Polyethyleneimine 1.5 1.5% 1.5 Bis-Tris Propane pH 6.5 100 mM PEG 400 1.5% C12E9  0.2% 487 10.37 Na-Succinate pH 5.5 100 mM 2,2,2-Trifluoroethanol 10% LDAO 0.13% 488 5.4 Ethanol 20 20.0% 10 0 mM PEG 200 2% 489 9.57 Isopropanol 23.8 23.8% 11.9 CAPSO pH 9.5 50 mM 490 4.79 Isopropanol 8.2 8.2% 4.1 Na-Malonate pH 5.0 100 mM Dextran Sulfate 2.4% 491 7.18 PEG MME 2K 11 11.0% 11 Hepes pH 7.5 100 mM Ethylene Glycol 4.2% Triton X-100 0.54% 492 5.37 PEG 10K 22.6 22.6% 22.6 Na-Acetate pH 5.5 50 mM 2,2,2-Trifluoroethanol 1% 493 4.54 Ethylene Glycol 10.6 10.6% 5.3 TRIS-Maleate pH 4.5 50 mM 1,4 Butanediol 3.2% 494 4.86 PEG 300 42.6 42.6% 21.3 TRIS-Maleate pH 4.5 50 mM Strontium Chloride 0.074 M 495 8.03 Isopropanol 32.8 32.8% 16.4 Na—K-Phosphate pH 7.5 100 mM 496 8.36 PEG 400 18.6 18.6% 9.3 CAPSO pH 8.5 50 mM MPD 4.3% 497 6.09 Methanol 31 31.0% 15.5 TRIS-HCl pH 6.5 100 mM D (+) Glucose 2.8% 498 6.92 PEG 6K 32 32.0% 32 Na-Cacodylate pH 7.5 100 mM Xylitol 33% C12E9  0.2% 499 3.33 PEG 400 16.4 16.4% 8.2 0 mM 1,4 Dioxane 5% Cymal-3 0.84% 500 7.07 PEG 600 9 9.0% 9 Mopso pH 7.5 100 mM 501 8.12 Potassium 28.4 1.0 M 33.39468303 Hepes pH 7.5 100 mM Jeffamine M-600 1% Sodium Tartrate 502 6.43 Ammonium 2 0.1 M 1 TRIS-Maleate pH 6.5 50 mM Phosphate 503 5.53 Ammonium 6 0.5 M 9.295774648 Mopso pH 6.5 100 mM Citrate 504 7.72 Magnesium 1.21 1.3 M 26.62 Mopso pH 7.5 100 mM Ammonium Sulfate 0.168 M Sulfate 505 7.96 TRIS-HCl pH 7.5 100 mM Isopropanol 4% 506 4.04 Ammonium 11.4 2.5 M 12.46242604 Na-Acetate pH 4.5 50 mM Zinc Acetate 0.288 M Triton X-100  0.2% Nitrate 507 2.67 Ferric Chloride 2 0.4 M 22.22222222 Mopso pH 6.5 100 mM Hexahydrate 508 5.36 Ammonium 8.2 1.8 M 8.964201183 Na-Succinate pH 5.5 100 mM Ammonium Sulfate 0.14 M Nitrate 509 5.35 Ammonium 15 3.3 M 16.39792899 Na-Malonate pH 7.0 100 mM Nitrate 510 7.26 Sodium Chloride 0.38 0.4 M 3.9 Hepes pH 7.5 100 mM Calcium Chloride 0.048 M 511 7.38 Lithium Chloride 8.33 8.3 M 41.65 Bis-Tris Propane pH 8.5 100 mM Sodium Thiocyanate 0.12 M 512 3.6 Magnesium 0.976 1.7 M 42.5 Na-Malonate pH 5.0 100 mM Sodium Thiocyanate 0.05 M Chloride 513 7.22 Lithium Chloride 1.9 1.9 M 9.5 TRIS-HCl pH 7.5 100 mM Sodium Thiocyanate 0.16 M Triton X-100 0.82% 514 6.56 Sodium Acetate 61.79 1.0 M 5 Na-Cacodylate pH 4.5 100 mM 515 5.05 Potassium 53.6 2.8 M 35.44565842 TRIS-Maleate pH 5.5 50 mM Zinc Acetate 0.104 M C12E9  0.2% Chloride 516 7.37 Potassium 29.4 1.6 M 19.44220817 Hepes pH 7.5 100 mM Magnesium Chloride 0.144 M Chloride 517 10.07 Tri-Sodium Citrate 0.791 0.7 M 20.59895833 CAPSO pH 9.5 50 mM Sodium Iodide 0.208 M Dihydrate 518 3.9 Zinc Sulfate 50 1.8 M 45 0 mM Hexahydrate 519 4.1 Ammonium 2 2.0 M 28.57142857 Na-Malonate pH 5.0 100 mM Calcium Chloride 0.08 M Sulfate 520 5.9 Lithium Sulfate 53.8 1.8 M 43.75 Na-Cacodylate pH 6.5 100 mM Lithium Chloride 0.242 M Monohydrate 521 6.33 Sodium Sulfate 0.084 0.1 M 6.5625 Hepes pH 6.5 100 mM 522 5.72 Sodium Chloride 1.16 1.2 M 11.9 0 mM Cadmium Chloride 0.025 M 523 7.18 Potassium 0.6 0.0 M 0.705521472 TRIS-HCl pH 6.5 100 mM Sodium Tartrate 524 4.63 Magnesium 1.1 1.8 M 45 Na-Succinate pH 5.5 100 mM Chloride 525 7.6 Potassium 22.8 1.2 M 15.07763082 TRIS-HCl pH 7.5 100 mM Sodium Iodide 0.266 M Chloride 526 7.35 Ammonium 1.06 1.1 M 15.14285714 Mopso pH 7.5 100 mM Sulfate 527 6.55 TRIS-HCl pH 6.5 100 mM Calcium Chloride 0.075 M 528 9.87 Potassium 30 1.6 M 19.83898792 CAPSO pH 9.5 50 mM Chloride 529 4.25 Na-Citrate pH 4.5 50 mM Sodium Thiocyanate 0.276 M 530 8.42 Sodium Acetate 36.25 2.0 M 10 0 mM DMSO 0.6% 531 5.95 Sodium Formate 7.98 2.3 M 16.62984892 TRIS-Maleate pH 5.5 50 mM 532 6.92 Lithium Sulfate 20.6 0.8 M 18.7727825 TRIS-HCl pH 6.5 100 mM Monohydrate 533 7.78 Potassium 12 0.4 M 14.11042945 Bis-Tris Propane pH 6.5 100 mM 1,6 Hexanediol 1.32 M Sodium Tartrate 534 6.98 Magnesium 1.4 1.5 M 30.8 Bis-Tris Propane pH 7.5 100 mM Potassium Chloride 0.05 M Sulfate 535 5.54 Sodium 0.34 0.3 M 29.6 Na-Cacodylate pH 6.5 100 mM Isopropanol 4% Phosphate 536 1.55 Ferric Chloride 4 0.9 M 44.44444444 Na-Citrate pH 4.5 50 mM Hexahydrate 537 5.75 Ammonium 14 0.4 M 7 Na-Citrate pH 6.5 50 mM Cymal-3  0.2% Phosphate 538 5.93 Ammonium 14 3.1 M 15.30473373 Na-Cacodylate pH 6.5 100 mM Magnesium Chloride 0.186 M Nitrate 539 6.25 Ammonium 33.6 7.3 M 36.73136095 Na-K-Phosphate pH 6.5 100 mM Ethanol 2% Nitrate 540 9 Ammonium 5.4 5.4% 2.7 Bis-Tris Propane pH 8.5 100 mM Jeffamine M-600 1% Bromide 541 5.08 Magnesium 0.31 0.3 M 6.82 Na-Succinate pH 5.5 100 mM Sulfate 542 8.59 Potassium 54.8 2.9 M 36.23921794 TRIS-HCl pH 8.5 100 mM Sodium Iodide 0.01 M Chloride 543 4.62 Sodium 0.305 0.3 M 26.3 TRIS-HCl pH 6.5 100 mM Phosphate 544 7.75 Potassium 48.4 1.0 M 31.66666667 0 mM Sodium Iodide 0.158 M Sodium Tartrate 545 6.94 Sodium Acetate 66.96 1.5 M 7.5 Na-Cacodylate pH 4.5 100 mM Ammonium Sulfate 0.21 M Glycerol  8.2% 546 4.73 Ammonium 45.8 1.1 M 22.9 Na-Malonate pH 7.0 100 mM Sodium Chloride 0.11 M Phosphate 547 7.13 Ammonium 4.28922 4.3 M 29.8 Na-Cacodylate pH 4.5 100 mM Ammonium Sulfate 0.273 M Acetate 548 6.38 TRIS-HCl pH 6.5 100 mM Barium Chloride 0.066 M Glucopyranoside  0.7% 549 5.1 Magnesium 2.02 0.9 M 22.14912281 Na-Cacodylate pH 6.5 100 mM Chloride 550 3.43 Cadmium Sulfate 20 0.2 M 10 Na-Malonate pH 7.0 100 mM Monohydrate 551 5.59 Ammonium 2.29 2.3 M 32.71428571 Na-Cacodylate pH 5.5 100 mM Sodium Iodide 0.214 M Sulfate 552 8.76 Sodium Formate 7.98 2.3 M 16.62984892 CAPSO pH 8.5 50 mM 553 5.32 PEG 4K 10 10.0% 10 TRIS-Maleate pH 4.5 50 mM 554 5.06 Ammonium 39.32 2.2 M 44 Mopso pH 6.5 100 mM Citrate 555 6.24 Tri-Sodium Citrate 0.269 0.2 M 7.005208333 Na-Cacodylate pH 5.5 100 mM Sodium Thiocyanate 0.288 M Dihydrate 556 5.13 Lithium Sulfate 56 1.7 M 42.5 Na-Acetate pH 5.5 50 mM 1,6 Hexanediol 0.25 M Monohydrate 557 7.23 Ammonium 2.84934 2.8 M 19.8 Hepes pH 7.5 100 mM Cobaltus Chloride 0.033 M Acetate 558 8.17 Magnesium 0.91 1.8 M 45 Hepes pH 8.5 100 mM Chloride 559 5.96 PEG 4K 10 10.0% 10 Na-Succinate pH 7.5 100 mM 560 6.85 Sodium Formate 46.4 5.0 M 35.71428571 TRIS-Maleate pH 6.5 50 mM Glycerol   2% 561 7.53 Sodium Formate 25.26 6.0 M 42.85714286 Na-Succinate pH 7.5 100 mM Calcium Chloride 0.005 M 562 7.47 Na-Malonate pH 7.0 100 mM Cobaltus Chloride 0.05 M 563 3.15 Cadmium Sulfate 26 0.3 M 13 TRIS-Maleate pH 4.5 50 mM Cadmium Chloride 0.023 M Monohydrate 564 4.3 Na-Succinate pH 4.5 100 mM Sodium Thiocyanate 0.4 M 565 6.49 Magnesium 0.15 0.2 M 3.3 Bis-Tris Propane pH 7.5 100 mM Ethanol 4.6% Sulfate 566 4.27 Lithium Sulfate 41.8 1.5 M 38.09234508 Na-Acetate pH 4.5 50 mM Ethanol 4.8% Monohydrate 567 6.9 Ammonium 1.05 1.1 M 15 Bis-Tris Propane pH 8.5 100 mM Cobaltus Chloride 0.05 M Sulfate 568 5.3 Ammonium 8.95 0.7 M 13.86619718 Hepes pH 8.5 100 mM Ammonium Sulfate 0.182 M C12E9  0.2% Citrate 569 6.84 TRIS-Maleate pH 6.5 50 mM Potassium Chloride 0.2 M 570 8.46 Magnesium 0.48 0.5 M 10.56 Mopso pH 6.5 100 mM 1,6 Hexanediol 1.68 M Sulfate 571 5.99 Magnesium 0.96 1.0 M 25 TRIS-Maleate pH 6.5 50 mM Potassium Chloride 0.02 M Chloride 572 4.97 Ammonium 15.91 1.2 M 24.64929577 Na-Succinate pH 7.5 100 mM Citrate 573 6.22 Ferric Chloride 1 0.2 M 11.11111111 Bis-Tris Propane pH 7.5 100 mM 1,6 Hexanediol 1.6 M Hexahydrate 574 7.12 Potassium 55.6 2.9 M 36.76825762 Na-Cacodylate pH 7.5 100 mM Cesium Chloride 0.002 M C12E9  0.2% Chloride 575 7.05 Sodium Chloride 2.98 3.0 M 30.5 Na-K-Phosphate pH 8.5 100 mM Ammonium Sulfate 0.168 M 576 6.22 Ammonium 19.4 19.4% 9.7 Na-Cacodylate pH 5.5 100 mM Bromide 577 6.29 Lithium Sulfate 18.8 0.7 M 17.13244228 Na-Succinate pH 6.5 100 mM Monohydrate 578 6.35 Ammonium 1.05 1.1 M 15 Na-Cacodylate pH 4.5 100 mM Jeffamine M-600 2.8% Glucopyranoside 0.84% Sulfate 579 7.23 Amonium 9.8 2.1 M 10.71331361 Na-K-Phosphate pH 8.5 100 mM Nitrate 580 3.74 Na-Malonate pH 5.0 100 mM Cobaltus Chloride 0.063 M 581 7.43 Ammonium 10 10.0% 5 Na-K-Phosphate pH 7.5 100 mM Bromide 582 6.1 Ammonium 2 2.0 M 28.57142857 Na-Succinate pH 6.5 100 mM Zinc Acetate 0.002 M Sulfate 583 2.95 Nickel Chloride 20 3.6% 45 Na-Malonate pH 5.0 100 mM Hexahydrate 584 7.46 Potassium 34.2 1.2 M 40.21472393 Na-Cacodylate pH 7.5 100 mM Isopropanol 2% Sodium Tartrate 585 3.02 Magnesium 2.12 0.9 M 23.24561404 TRIS-Maleate pH 4.5 50 mM Acetonitrile 2.48% Chloride 586 4.02 Magnesium 0.86 1.2 M 30 Na-Acetate pH 4.5 50 mM Jeffamine M-600 1% Chloride 587 6.98 Tri-Sodium Citrate 0.238 0.2 M 6.197916667 Mopso pH 7.5 100 mM Cupric Chloride 0.028 M Glucopyranoside  0.8% Dihydrate 588 4.78 Nickel Chloride 58 2.0% 25 CAPSO pH 9.5 50 mM DMSO 1.02% Glycerol  3.6% Hexahydrate 589 7.52 Mopso pH 7.5 100 mM Cobaltus Chloride 0.041 M 590 5.09 Ammonium 34.77 1.8 M 36 TRIS-Maleate pH 4.5 50 mM Acetonitrile 3.52% Citrate 591 6.79 Na-K-Phosphate pH 6.5 100 mM Sodium Iodide 0.4 M 592 7.3 Lithium Chloride 8.49 8.5 M 42.45 Hepes pH 8.5 100 mM Isopropanol 2% 593 9.2 Ammonium 6.6 6.6% 3.3 CAPSO pH 8.5 50 mM Potassium Chloride 0.046 M Bromide 594 6.82 Hepes pH 6.5 100 mM Urea 0.12 M 595 7.45 Ammonium 12 2.6 M 13.1183432 CAPSO pH 8.5 50 mM Potassium Chloride 0.168 M Nitrate 596 5.54 Calcium Acetate 40.4 0.5 M 26.93333333 Na-Acetate pH 4.5 50 mM Potassium Chloride 0.112 M Dihydrate 597 5.23 Ammonium 2 2.0 M 28.57142857 TRIS-Maleate pH 5.5 50 mM Sulfate 598 8.04 Ammonium 1.9266 1.9 M 13.4 TRIS-HCl pH 8.5 100 mM Ammonium Sulfate 0.28 M Acetate 599 8.09 Potassium 10.4 0.4 M 12.22903885 Hepes pH 8.5 100 mM Sodium Tartrate 600 6.07 Hepes pH 6.5 100 mM Cadmium Chloride 0.02 M 601 4.77 Sodium Chloride 2.88 2.9 M 29.5 Na-Succinate pH 5.5 100 mM Cadmium Chloride 0.023 M 602 9.12 Sodium Sulfate 0.44 0.7 M 34.375 Bis-Tris Propane pH 7.5 100 mM Jeffamine M-600 3.8% 603 6.33 Ammonium 37.4 8.2 M 40.88550296 TRIS-HCl pH 6.5 100 mM Barium Chloride 0.002 M Nitrate 604 7.12 Ammonium 1.06 1.1 M 15.14285714 Bis-Tris Propane pH 7.5 100 mM Sulfate 605 6.3 Ammonium 50 9.0 M 45 Mopso pH 6.5 100 mM Nitrate 606 5.2 Ammonium 14.77 0.9 M 18 TRIS-Maleate pH 5.5 50 mM Ethanol 2.8% Citrate 607 4.82 Ammonium 30 6.6 M 32.79585799 Na-Citrate pH 6.5 50 mM Maltoside 0.88% Nitrate 608 5.29 Ammonium 10 10.0% 5 TRIS-Maleate pH 4.5 50 mM Bromide 609 5.08 Ammonium 26.82 2.1 M 41.55211268 Mopso pH 7.5 100 mM Glycerol   3% Citrate 610 5.16 Ammonium 27.05 1.5 M 30 Hepes pH 8.5 100 mM Cesium Chloride 0.118 M Citrate 611 6.99 Sodium Acetate 40 1.3 M 6.25 Na—K-Phosphate pH 6.5 100 mM 612 5.22 Na-Acetate pH 5.5 50 mM Calcium Chloride 0.021 M 613 8.09 Tri-Sodium Citrate 0.188 0.2 M 4.895833333 Na—K-Phosphate pH 7.5 100 mM Dihydrate 614 7.89 Sodium 0.349 0.3 M 30.1 TRIS-Maleate pH 6.5 50 mM Phosphate 615 4.29 Sodium Sulfate 0.192 0.3 M 15 Na-Malonate pH 7.0 100 mM Zinc Acetate 0.204 M 616 7.21 Na—K-Phosphate pH 6.5 100 mM Triton X-100 0.52% 617 7.62 Potassium 58.4 1.2 M 40 Na-Succinate pH 7.5 100 mM Sodium Tartrate 618 7.96 Ammonium 2.93 3.1 M 44.28571429 Na—K-Phosphate pH 7.5 100 mM Potassium Chloride 0.002 M Sulfate 619 6.68 Calcium Chloride 2.14 0.8 M 20.18867925 Hepes pH 6.5 100 mM 620 6.25 Sodium Formate 32.82 5.5 M 39.28571429 Na—K-Phosphate pH 5.5 100 mM Sodium Thiocyanate 0.14 M 621 8.83 Na—K-Phosphate pH 8.5 100 mM Potassium Chloride 0.144 M Triton X-100  0.2% 622 5.22 Na-Cacodylate pH 5.5 100 mM Phenol 0.06 M 623 7.01 Na—K-Phosphate pH 6.5 100 mM Cesium Chloride 0.4 M 624 4.84 Ammonium 25 1.9 M 38.73239437 Na—K-Phosphate pH 6.5 100 mM Citrate 625 7.35 Na-Succinate pH 7.5 100 mM Barium Chloride 0.009 M Glucopyranoside 0.46% 626 4.91 Sodium Chloride 2.18 2.2 M 22.3 Na-Cacodylate pH 5.5 100 mM Sodium Thiocyanate 0.24 M 627 7.56 Magnesium 1.27 1.4 M 27.94 Hepes pH 6.5 100 mM Jeffamine M-600 2.7% Sulfate 628 7.83 Sodium 0.322 0.3 M 27.8 TRIS-Maleate pH 6.5 50 mM Phosphate 629 6.77 Magnesium 3.16 1.4 M 34.64912281 Bis-Tris Propane pH 6.5 100 mM Cupric Chloride 0.005 M Chloride 630 5.82 Ammonium 1.05 1.1 M 15 Tris-Maleate pH 5.5 50 mM Sulfate 631 7.1 Calcium Chloride 2.03 0.8 M 19.1509434 Hepes pH 7.5 100 mM Sodium Chloride 0.4 M 632 4.32 Nickel Chloride 21.6 0.5% 6.25 TRIS-Maleate pH 6.5 50 mM Glucopyranoside  0.8% Hexahydrate 633 8.29 Magnesium 2.01 0.9 M 22.03947368 Bis-Tris Propane pH 8.5 100 mM Chloride 634 6.24 Calcium Acetate 46.2 0.6 M 30.8 TRIS-Maleate pH 6.5 50 mM Sodium Iodide 0.116 M Dihydrate 635 8.99 Potassium 1.47 0.4 M 12.76041667 Bis-Tris Propane pH 7.5 100 mM Potassium Chloride 0.12 M Cymal-3  0.8% Phosphate 636 6.67 Potassium 31.8 1.1 M 37.39263804 Na-Citrate pH 6.5 50 mM Cadmium Chloride 0.002 M Glycerol   1% Sodium Tartrate 637 8.51 Lithium Sulfate 29 1.1 M 26.42770352 Na-Malonate pH 5.0 100 mM Acetonitrile 2% Monohydrate 638 8.49 Calcium Acetate 20 0.3 M 13.33333333 CAPSO pH 8.5 50 mM Dihydrate 639 9.02 Potassium 2.71 0.7 M 23.52430556 Na-Acetate pH 5.5 50 mM Sodium Fluoride 0.3104 M Phosphate 640 5.81 Lithium Chloride 5.33 5.3 M 26.65 TRIS-HCl pH 6.5 100 mM 1,6 Hexanediol 1.48 M Glucopyranoside  0.2% 641 6.77 Potassium 6.4 0.2 M 7.525562372 TRIS-Maleate pH 6.5 50 mM Sodium Tartrate 642 8.19 TRIS-HCl pH 8.5 100 mM Taurine 0.06 M 643 9.14 Potassium 40.2 2.1 M 26.58424382 CAPSO pH 8.5 50 mM 1,6 Hexanediol 1.36 M Chloride 644 7.47 Sodium Acetate 70 1.8 M 8.75 Na-Citrate pH 4.5 50 mM Cobaltus Chloride 0.025 M Glycerol  4.2% 645 8.04 Ammonium 1.24 1.2 M 17.71428571 Hepes pH 8.5 100 mM DMSO 2.4% Sulfate 646 6.43 Ammonium 18 3.9 M 19.67751479 TRIS-HCl pH 6.5 100 mM Calcium Chloride 0.068 M Nitrate 647 7.63 Ammonium 0.763542 0.8 M 5.3 CAPSO pH 9.5 50 mM Cadmium Chloride 0.019 M Acetate 648 6.2 Calcium Acetate 28.6 0.4 M 19.06666667 Bis-Tris Propane pH 9.5 100 mM Isopropanol 2% Dihydrate 649 6.45 Ammonium 7.6 0.2 M 3.8 Na-Malonate pH 7.0 100 mM DMSO 2.52% Phosphate 650 7.7 Ammonium 3.16368 3.2 M 22 CAPSO pH 9.5 50 mM Barium Chloride 0.026 M LDAO 0.28% Acetate 651 8.49 Lithium Sulfate 36.2 1.3 M 32.9890644 Hepes pH 8.5 100 mM Sodium Iodide 0.01 M Monohydrate 652 5.5 Sodium Formate 20.01 5.8 M 41.69965875 Na-Citrate pH 4.5 50 mM Cupric Chloride 0.005 M 653 5.37 Na-Succinate pH 5.5 100 mM Taurine 0.06 M 654 7.22 Sodium Acetate 26.96 2.3 M 11.25 Na-Citrate pH 6.5 50 mM Isopropanol 2% 655 6.19 Ammonium 1.05 1.1 M 15 Na—K-Phosphate pH 5.5 100 mM Sulfate 656 6.91 Ammonium 1.00386 1.0 M 7 Bis-Tris Propane pH 6.5 100 mM Calcium Chloride 0.02 M Acetate 657 4.72 Magnesium 1.4 1.5 M 30.8 Na-Succinate pH 5.5 100 mM Sulfate 658 5.59 Ammonium 2.93 3.2 M 45.71428571 0 mM Sulfate 659 7.33 Magnesium 1.73 0.8 M 18.96929825 TRIS-Maleate pH 5.5 50 mM Jeffamine M-600 3.6% Chloride 660 4.32 Ammonium 10.4 10.4% 5.2 Na-Succinate pH 4.5 100 mM Barium Chloride 0.038 M Bromide 661 8.93 Magnesium 0.54 1.4 M 35 CAPSO pH 8.5 50 mM Chloride 662 8.99 CAPSO pH 8.5 50 mM Barium Chloride 0.073 M 663 7.75 Potassium 39 1.3 M 41.66666667 Na-Succinate pH 7.5 100 mM Sodium Tartrate 664 7.16 Ammonium 26 26.0% 13 Bis-Tris Propane pH 6.5 100 mM Isopropanol 2% Bromide 665 5.28 Ammonium 25.2 0.6 M 12.6 Na—K-Phosphate pH 7.5 100 mM Phosphate 666 8.74 Ammonium 2.5 2.5 M 35.71428571 Na—K-Phosphate pH 8.5 100 mM Sulfate 667 1.7 Ferric Chloride 1.5 0.3 M 16.66666667 TRIS-Maleate pH 6.5 50 mM Sodium Chloride 0.39 M Hexahydrate 668 7.13 Na—K-Phosphate pH 7.5 100 mM Sodium Thiocyanate 0.4 M 669 5.87 Ammonium 1 1.0 M 14.28571429 Na-Malonate pH 7.0 100 mM Sulfate 670 8.74 Na—K-Phosphate pH 8.5 100 mM Sodium Thiocyanate 0.128 M 671 8.15 Lithium Chloride 5.33 5.3 M 26.65 Hepes pH 8.5 100 mM 672 7.14 Na—K-Phosphate pH 6.5 100 mM Potassium Chloride 0.068 M 673 7.28 Ammonium 24.4 24.4% 12.2 Mopso pH 7.5 100 mM Cesium Chloride 0.122 M Bromide 674 3.44 Zinc Sulfate 25 1.4 M 34.03465347 Na-Malonate pH 5.0 100 mM Sodium Thiocyanate 0.212 M Hexahydrate 675 9.46 Calcium Chloride 0.75 0.3 M 7.075471698 CAPSO pH 9.5 50 mM Sodium Chloride 0.33 M LDAO 0.42% 676 1.86 Ferric Chloride 4 0.9 M 44.44444444 Na-Citrate pH 5.5 50 mM Cupric Chloride 0.002 M Hexahydrate 677 4.36 Sodium Chloride 1.85 1.9 M 19 Na-Acetate pH 4.5 50 mM 678 6.12 Calcium Chloride 2.86 1.1 M 26.98113208 TRIS-HCl pH 7.5 100 mM Magnesium Chloride 0.242 M 679 1.37 Ferric Chloride 3.5 0.8 M 38.88888889 0 mM Jeffamine M-600 4.5% Glycerol   4% Hexahydrate 680 7.23 Ammonium 5.21196 5.2 M 36.2 0 mM Acetate 681 10.09 Ammonium 0.84 0.8 M 12 CAPSO pH 9.5 50 mM Sodium Fluoride 0.1856 M Sulfate 682 6.69 Na-Citrate pH 6.5 50 mM Calcium Chloride 0.022 M C12E9  0.2% 683 9.1 Lithium Sulfate 43.6 1.6 M 39.7326853 CAPSO pH 8.5 50 mM Isopropanol 2% Monohydrate 684 4.66 Zinc Sulfate 31.8 1.7 M 43.29207921 Na-Acetate pH 5.5 50 mM Sodium Iodide 0.01 M Hexahydrate 685 6.58 Bis-Tris Propane pH 8.5 100 mM Zinc Acetate 0.11 M 686 7.26 Ammonium 4.1067 4.1 M 28.5 Bis-Tris Propane pH 6.5 100 mM Barium Chloride 0.06 M Glycerol  4% Acetate 687 8.63 Bis-Tris Propane pH 8.5 100 mM Ethanol 3.8% 688 6.21 0 mM Cobaltus Chloride 0.18 M 689 6.98 Ammonium 2.56542 2.6 M 17.8 Bis-Tris Propane pH 9.5 100 mM Acetate 690 3.99 Ammonium 27.8 0.7 M 13.9 CAPSO pH 8.5 50 mM Magnesium Chloride 0.222 M Cymal-3  0.2% Phosphate 691 6.42 Bis-Tris Propane pH 6.5 100 mM Sodium Iodide 0.156 M 692 5.8 Calcium Acetate 26.2 0.3 M 17.46666667 Na-Cacodylate pH 4.5 100 mM Ethanol 4.2% Dihydrate 693 4.8 Magnesium 1.56 1.7 M 34.32 Na-Acetate pH 5.5 50 mM Acetonitrile 1.44% Sulfate 694 7.23 Na-Succinate pH 7.5 100 mM Guanidine HCl 0.2 M 695 4.3 Sodium Sulfate 0.131 0.2 M 10.234375 Na-Cacodylate pH 4.5 100 mM Cobaltus Chloride 0.038 M 696 8.49 Bis-Tris Propane pH 8.5 100 mM Sodium Iodide 0.1 M 697 4.79 Magnesium 1.3 0.6 M 14.25438596 Bis-Tris Propane pH 9.5 100 mM Lithium Sulfate 0.21 M Chloride 698 3.45 Magnesium 1.39 0.6 M 15.24122807 Na-Acetate pH 4.5 50 mM Cadmium Chloride 0.022 M Chloride 699 3.7 Cadmium Sulfate 15.2 0.2 M 7.6 Na-Cacodylate pH 4.5 100 mM Potassium Chloride 0.094 M Monohydrate 700 7.32 Ammonium 3.78222 3.8 M 26.3 TRIS-Maleate pH 6.5 50 mM Acetate 701 7.82 Potassium 38.4 1.0 M 33.33333333 Hepes pH 7.5 100 mM Acetonitrile 1.92% Sodium Tartrate 702 5.77 0 mM Cadmium Chloride 0.013 M Cymal-3  0.3% 703 5.73 Lithium Chloride 8 8.0 M 40 TRIS-HCl pH 7.5 100 mM 704 5.81 Magnesium 1.57 0.7 M 17.21491228 Mopso pH 6.5 100 mM Chloride 705 4.43 Sodium Sulfate 0.407 0.6 M 31.796875 Na-Acetate pH 4.5 50 mM Glycerol  4.6% 706 4.26 Zinc Sulfate 26.4 1.4 M 35.94059406 0 mM Cesium Chloride 0.02 M Hexahydrate 707 8.61 Potassium 0.92 0.2 M 7.986111111 TRIS-Maleate pH 6.5 50 mM Phosphate 708 8.86 CAPSO pH 8.5 50 mM Magnesium Chloride 0.298 M 709 8.64 Potassium 23 0.8 M 27.04498978 CAPSO pH 8.5 50 mM Sodium Tartrate 710 6.69 Potassium 22 0.8 M 25.86912065 TRIS-HCl pH 7.5 100 mM Sodium Tartrate 711 3.48 Lithium Chloride 2.52 2.5 M 12.6 Na-Malonate pH 5.0 100 mM DMSO 1.68% 712 5.28 Ammonium 6 0.5 M 9.295774648 Na-Citrate pH 5.5 50 mM Acetonitrile 3.28% Citrate 713 8.05 Na—K-Phosphate pH 7.5 100 mM 1,6 Hexanediol 0.96 M 714 5.56 Sodium 0.29 0.3 M 25 CAPSO pH 9.5 50 mM Ammonium Sulfate 0.266 M Phosphate 715 2.96 Calcium Chloride 1.38 0.5 M 13.01886792 TRIS-Maleate pH 4.5 50 mM 716 5.47 Calcium Chloride 1.43 0.5 M 13.49056604 Mopso pH 6.5 100 mM Magnesium Chloride 0.206 M Maltoside  0.8% 717 6.12 Nickel Chloride 13.8 0.0% 0.25 Na-Cacodylate pH 7.5 100 mM Lithium Chloride 0.426 M Hexahydrate 718 4.37 Potassium 56.4 3.0 M 37.2972973 Na-Acetate pH 4.5 50 mM Chloride 719 3.95 Lithium Chloride 1.13 1.1 M 5.65 Na-Cacodylate pH 4.5 100 mM 720 7.73 Sodium Sulfate 0.37 0.6 M 28.90625 Bis-Tris Propane pH 6.5 100 mM 721 6.13 Ammonium 1.65 1.7 M 23.57142857 0 mM Sodium Iodide 0.228 M Sulfate 722 4.1 Magnesium 1.15 1.3 M 25.3 Na-Succinate pH 4.5 100 mM Sodium Thiocyanate 0.192 M Sulfate 723 6.1 Ammonium 1.78 1.8 M 25.42857143 Na-Citrate pH 6.5 50 mM Sulfate 724 6.72 Sodium Chloride 1.86 1.9 M 19.1 Bis-Tris Propane pH 6.5 100 mM DMSO 1.32% 725 5.18 Ammonium 51.6 6.2 M 31 0 mM Ethanol 4.6% Nitrate 726 1.54 Ferric Chloride 3.1 0.7 M 34.44444444 TRIS-Maleate pH 5.5 50 mM Hexahydrate 727 9.83 Sodium Chloride 1.57 1.6 M 16.1 Hepes pH 8.5 100 mM Jeffamine M-600 2.5% 728 3.07 Cadmium Sulfate 36.8 0.4 M 18.4 TRIS-Maleate pH 4.5 50 mM Ammonium Sulfate 0.168 M Monohydrate 729 6.79 Ammonium 0.9633 1.0 M 6.7 Na-Succinate pH 6.5 100 mM Acetate 730 7.97 Ammonium 19.6 4.3 M 21.42662722 TRIS-HCl pH 8.5 100 mM Sodium Fluoride 0.2224 M Nitrate 731 5.39 Ammonium 20 4.4 M 21.86390533 TRIS-Maleate pH 5.5 50 mM Nitrate 732 4.79 Ammonium 32.05 2.1 M 42 Na-Cacodylate pH 5.5 100 mM Citrate 733 7.46 Ammonium 2.6 2.6 M 37.14285714 Na-Succinate pH 7.5 100 mM Sulfate 734 8.05 Na—K-Phosphate pH 7.5 100 mM 1,6 Hexanediol 1.44 M Glycerol  4.2% 735 5.48 Nickel Chloride 20 1.0% 12.5 TRIS-HCl pH 8.5 100 mM Barium Chloride 0.005 M Hexahydrate 736 2.07 Nickel Chloride 46 1.5% 18.75 Na-Citrate pH 5.5 50 mM Barium Chloride 0.08 M Glucopyranoside 0.88% Hexahydrate 737 10.78 Potassium 15 0.5 M 17.63803681 0 mM Jeffamine M-600 1% Sodium Tartrate 738 6.99 Lithium Sulfate 44.8 1.6 M 40.82624544 Na-Succinate pH 7.5 100 mM Monohydrate 739 4.83 Ammonium 5.4 0.1 M 2.7 TRIS-HCl pH 6.5 100 mM Phosphate 740 8.32 Tri-Sodium Citrate 0.86 0.7 M 22.39583333 TRIS-HCl pH 7.5 100 mM Sodium Iodide 0.132 M Dihydrate 741 7.84 Sodium Acetate 11.43 2.5 M 12.5 0 mM 742 9.1 Tri-Sodium Citrate 0.995 0.8 M 25.91145833 Mopso pH 6.5 100 mM Isopropanol 4.6% Dihydrate 743 7.35 Sodium Formate 43.53 3.4 M 23.92857143 Bis-Tris Propane pH 6.5 100 mM 744 7.53 Sodium Acetate 56.61 2.8 M 13.75 TRIS-HCl pH 6.5 100 mM 745 7.22 Na-Succinate pH 7.5 100 mM Glycine 0.2 M 746 6.56 Mopso pH 6.5 100 mM Betaine Monohydrate 0.12 M 747 7.29 Potassium 59.6 3.2 M 39.41345601 Na-Succinate pH 7.5 100 mM Chloride 748 7.05 Ammonium 4.43118 4.4 M 30.8 TRIS-Maleate pH 5.5 50 mM Acetate 749 5.99 Nickel Chloride 20 3.5% 43.75 Na-Succinate pH 7.5 100 mM Hexahydrate 750 7.95 Sodium Chloride 0.86 0.9 M 8.8 Bis-Tris Propane pH 7.5 100 mM Calcium Chloride 0.07 M 751 4.39 Sodium Sulfate 0.115 0.2 M 8.984375 TRIS-Maleate pH 5.5 50 mM Yttrium Chloride 0.08 M 752 4.9 Lithium Chloride 0.77 0.8 M 3.85 Na-Cacodylate pH 5.5 100 mM 1,4 Butanediol 3.1% 753 6.45 Na-Succinate pH 6.5 100 mM Glycyl-Glycyl-Glycine 0.018 M Triton X-100 0.78% 754 5.17 Ammonium 52.6 9.0 M 45 0 mM Nitrate 755 8.18 Hepes pH 8.5 100 mM Dextran Sulfate 1.8% 756 7.1 Potassium 50 2.6 M 33.06497987 Na-Citrate pH 6.5 50 mM PEG 400 1.8% Chloride 757 7.07 Ammonium 2.92032 2.9 M 20.3 Na—K-Phosphate pH 6.5 100 mM Xylitol 3.2% Acetate 758 5.19 Na-Cacodylate pH 5.5 100 mM Xylitol 3.7% Triton X-100 0.24% 759 5.28 Ammonium 20 20.0% 10 Na-Citrate pH 5.5 50 mM Bromide 760 6.92 Ammonium 32.6 7.1 M 35.63816568 Na—K-Phosphate pH 8.5 100 mM Xylitol 4.1% Nitrate 761 6.26 Lithium Sulfate 10 0.4 M 9.113001215 Na—K-Phosphate pH 6.5 100 mM Monohydrate 762 7.1 Ammonium 2.98116 3.0 M 20.7 Hepes pH 6.5 100 mM Strontium Chloride 0.066 M Acetate 763 8.23 Sodium Chloride 1.95 2.0 M 20 Mopso pH 7.5 100 mM 764 7.27 Na—K-Phosphate pH 6.5 100 mM Phenol 0.025 M 765 8.52 Lithium Sulfate 8 0.3 M 7.290400972 Na-Malonate pH 5.0 100 mM 1,4 Butanediol 1.5% Monohydrate 766 6.95 Potassium 37.4 1.3 M 43.97750511 Hepes pH 6.5 100 mM 1,4 Dioxane 0.5% Sodium Tartrate 767 5.39 Zinc Sulfate 5 0.3 M 6.806930693 Bis-Tris Propane pH 6.5 100 mM Glycine 0.3 M Hexahydrate 768 5.42 Ammonium 1.71 1.7 M 24.42857143 TRIS-Maleate pH 5.5 50 mM Sulfate 769 10.07 Ammonium 1.24 1.2 M 17.71428571 CAPSO pH 9.5 50 mM Guanidine HCl 0.054 M Triton X-100 0.92% Sulfate 770 4.94 Mopso pH 6.5 100 mM Ethyl Acetate 2.55% 771 4.39 Ammonium 1.65 1.7 M 23.57142857 TRIS-Maleate pH 4.5 50 mM sec-butanol 4.7% Sulfate 772 6.99 Ammonium 2.5 2.5 M 35.71428571 TRIS-Maleate pH 6.5 50 mM 1,4 Butanediol 2.7% Sulfate 773 0.86 Nickel Chloride 43.6 1.8% 21.875 0 mM Dextran Sulfate 1.86% Hexahydrate 774 8.36 Magnesium 2.79 1.2 M 30.59210526 CAPSO pH 8.5 50 mM MPD 0.5% Glucopyranoside  0.2% Chloride 775 6.03 Calcium Chloride 2.03 0.8 M 19.1509434 Na-Cacodylate pH 7.5 100 mM 1,4 Dioxane 2.8% 776 7.28 Na-Citrate pH 5.5 50 mM Taurine 0.12 M 777 3.68 Calcium Chloride 1.63 0.6 M 15.37735849 Na-Cacodylate pH 4.5 100 mM 778 8.15 Ammonium 1 1.0 M 14.28571429 TRIS-HCl pH 8.5 100 mM Trimethylamine HCl 0.091 M Sulfate 779 5.96 Sodium Acetate 11.8 1.5 M 7.5 Na-Malonate pH 7.0 100 mM PEG 400 0.6% 780 6.76 Potassium 21.6 0.8 M 25.39877301 Hepes pH 6.5 100 mM Trimethylamine HCl 0.018 M Sodium Tartrate 781 6.09 Tri-Sodium Citrate 0.319 0.3 M 8.307291667 Na-Malonate pH 5.0 100 mM Ethylene Glycol 2.7% Dihydrate 782 7.42 Sodium Acetate 46.07 2.0 M 10 TRIS-HCl pH 6.5 100 mM 783 6.27 Na—K-Phosphate pH 5.5 100 mM Triton X-100 0.74% 784 5.52 Calcium Acetate 31.6 0.4 M 21.06666667 Na-Acetate pH 4.5 50 mM 2,2,2-Trifluoroethanol 1.6% Dihydrate 785 7.06 Hepes pH 8.5 100 mM Yttrium Chloride 0.002 M 786 3.99 Cadmium Sulfate 25.6 0.3 M 12.8 TRIS-Maleate pH 5.5 50 mM D (+) Glucose 4.5% Monohydrate 787 6.13 Sodium Formate 20 4.3 M 30.35714286 Na-Acetate pH 4.5 50 mM 2,2,2-Trifluoroethanol 1.6% 788 7.76 Sodium Formate 15 4.4 M 31.25911451 Na—K-Phosphate pH 7.5 100 mM 789 5.42 Na-Cacodylate pH 5.5 100 mM Acetonitrile 8% 790 5.86 Zinc Sulfate 30 1.6 M 40.84158416 Na-Cacodylate pH 7.5 100 mM PEG 400 3.9% Hexahydrate 791 7.15 Na-Cacodylate pH 7.5 100 mM Dextran Sulfate 1.44% 792 9.02 Potassium 0.44 0.1 M 3.819444444 0 mM Guanidine HCl 0.04 M Phosphate 793 6.53 Sodium Sulfate 0.289 0.5 M 22.578125 Hepes pH 6.5 100 mM Phenol 0.037 M 794 7.15 Sodium Formate 41.29 6.0 M 42.85714286 Na-Cacodylate pH 7.5 100 mM Glycerol   2% 795 6.32 Sodium Acetate 10.36 2.1 M 10.625 CAPSO pH 8.5 50 mM Dextran Sulfate 3% 796 5.92 Sodium Chloride 0.98 1.0 M 10 Na-Cacodylate pH 5.5 100 mM 797 6.21 Ammonium 14.4 3.1 M 15.74201183 Na-Succinate pH 6.5 100 mM Nitrate 798 4.38 Magnesium 2.34 1.0 M 25.65789474 Na-Acetate pH 5.5 50 mM Taurine 0.052 M Chloride 799 9.24 Potassium 1.59 0.4 M 13.80208333 0 mM Phosphate 800 6.77 Sodium Formate 34.71 5.6 M 40 Hepes pH 6.5 100 mM Yttrium Chloride 0.02 M 801 7.73 Ammonium 23.4 5.1 M 25.58076923 CAPSO pH 9.5 50 mM 2,2,2-Trifluoroethanol 4.1% Glycerol  6.4% Nitrate 802 8.33 Potassium 5.8 0.2 M 6.8200409 Hepes pH 8.5 100 mM 1,4 Dioxane 1.5% Sodium Tartrate 803 4.54 Na-Citrate pH 4.5 50 mM 1,4-Dithio-DL-Threitol 0.021 M 804 6.66 Ammonium 15.6 15.6% 7.8 Na—K-Phosphate pH 6.5 100 mM Bromide 805 4.55 Na-Citrate pH 4.5 50 mM Glycerol Anhydrous 3.4% Cymal-3  0.4% 806 5.08 Ammonium 20.68 1.6 M 32.03943662 0 mM Dextran Sulfate 2.04% Citrate 807 6.96 Potassium 25 0.9 M 29.39672802 Na-Succinate pH 6.5 100 mM Betaine Monohydrate 0.002 M Sodium Tartrate 808 6.34 Sodium Acetate 33.39 1.6 M 8.15 Na-Acetate pH 5.5 50 mM Phenol 0.05 M 809 7.02 Ammonium 25.6 5.6 M 27.98579882 Na—K-Phosphate pH 8.5 100 mM 1,4 Dioxane 5% Nitrate 810 4.47 Cadmium Sulfate 47 0.5 M 23.5 TRIS-Maleate pH 6.5 50 mM Glycine 0.22 M Monohydrate 811 5.85 Nickel Chloride 5 2.2% 27.5 0 mM 2,2,2-Trifluoroethanol 1.2% Hexahydrate 812 4.06 Magnesium 0.9 1.0 M 19.8 Na-Cacodylate pH 4.5 100 mM 2,2,2-Trifluoroethanol 2% Sulfate 813 9.94 Ammonium 1.78 1.8 M 25.42867143 CAPSO pH 9.5 50 mM Ethylene Glycol 1.2% Sulfate 814 7.4 Tri-Sodium Citrate 0.68 0.6 M 17.70833333 Bis-Tris Propane pH 6.6 100 mM Betaine Monohydrate 0.03 M Dihydrate 815 5.04 Ammonium 44.8 1.1 M 22.4 Na-Acetate pH 5.5 50 mM 1,4 Dioxane 3.5% Phosphate 816 5.19 Na-Malonate pH 7.0 100 mM Guanidine HCl 0.02 M 817 5.53 Ammonium 0.84 0.8 M 12 Na-Malonate pH 7.0 100 mM Guanidine HCl 0.02 M Sulfate 818 6.6 Sodium Acetate 75.18 1.3 M 6.25 Na-Citrate pH 4.5 50 mM Xylitol 1.2% 819 8.98 Calcium Chloride 2.27 0.9 M 21.41509434 0 mM Glycerol Anhydrous 1% 820 7.76 Sodium Sulfate 0.26 0.4 M 20.3125 Na-Citrate pH 4.5 50 mM 821 1.41 Ferric Chloride 3.9 0.9 M 43.33333333 Na-Citrate pH 4.5 50 mM 1,4 Butanediol 1% Hexahydrate 822 5.26 Zinc Sulfate 18 1.0 M 24.5049505 TRIS-Maleate pH 6.5 50 mM Yttrium Chloride 0.025 M Hexahydrate 823 4.62 Mopso pH 6.5 100 mM Urea 0.18 M 824 6.59 Sodium Sulfate 0.247 0.4 M 19.296875 Na-Succinate pH 7.5 100 mM Dextran Sulfate 1.56% 825 5.89 Sodium Acetate 19.11 1.8 M 9 TRIS-Maleate pH 5.5 50 mM ATP disodium salt 0.018 M Triton X-100 0.22% 826 8.19 Potassium 1.73 0.5 M 15.01736111 Hepes pH 6.5 100 mM Phosphate 827 7.76 Magnesium 0.91 1.5 M 37.5 TRIS-HCl pH 7.5 100 mM Chloride 828 6.86 Sodium Acetate 41.43 2.3 M 11.5 Hepes pH 6.5 100 mM 829 8.38 Potassium 48.4 2.6 M 32.00690052 Hepes pH 8.5 100 mM Strontium Chloride 0.023 M Glucopyranoside 0.76% Chloride 830 8.6 Na—K-Phosphate pH 8.5 100 mM 1,4 Dioxane 2% 831 6.28 Ammonium 1.81 1.8 M 25.85714286 Na-Cacodylate pH 5.5 100 mM Sulfate 832 5.4 Sodium Acetate 15.36 1.9 M 9.5 Na-Citrate pH 4.5 50 mM Trimethylamine HCl 0.092 M 833 8.57 Lithium Sulfate 40 1.5 M 36.45200486 TRIS-HCl pH 7.5 50 mM Monohydrate 834 7.26 Ammonium 4.07628 4.1 M 28.3 TRIS-HCl pH 6.5 100 mM PEG 400 5% Acetate 835 4.49 Ammonium 31.6 6.9 M 34.54497041 0 mM MPD 3.3% C12E9  0.2% Nitrate 836 7.63 Na—K-Phosphate pH 7.5 100 mM Guanidine HCl 0.36 M 837 5.62 Lithium Sulfate 20 0.7 M 18.22600243 Hepes pH 7.5 100 mM Glycerol Anhydrous 1% Monohydrate 838 7.82 Potassium 20 0.7 M 23.51738241 TRIS-HCl pH 7.5 100 mM Sodium Tartrate 839 8.28 Lithium Sulfate 31.6 1.2 M 28.79708384 Hepes pH 8.5 100 mM Trimethylamine HCl 0.046 M Glucopyranoside 0.56% Monohydrate 840 8.08 Potassium 21.4 0.8 M 25.16359918 Hepes pH 8.5 100 mM Sodium Tartrate 841 6.92 Ammonium 1.81 1.8 M 25.85714286 Na-Succinate pH 7.5 100 mM Sulfate 842 6.75 Potassium 13.6 0.5 M 15.99182004 Mopso pH 6.5 100 mM Ethylene Glycol 2.5% Sodium Tartrate 843 6.65 Ammonium 54.8 6.0 M 30 CAPSO pH 8.5 50 mM Yttrium Chloride 0.002 M Nitrate 844 5.96 Ammonium 0.84 0.8 M 12 Na—K-Phosphate pH 5.5 100 mM sec-butanol 1% Glucopyranoside  0.6% Sulfate 845 6.29 Calcium Chloride 3.75 1.4 M 35.37735849 0 mM Glycyl-Glycyl-Glycine 0.002 M Cymal-3 0.74% 846 6.52 Hepes pH 6.5 100 mM Betaine Monohydrate 0.082 M 847 7.01 Sodium Sulfate 0.115 0.2 M 8.984375 Na-Cacodylate pH 7.5 100 mM Guanidine HCl 0.09 M 848 5.92 Ammonium 16 0.4 M 8 Bis-Tris Propane pH 8.5 100 mM Phosphate 849 6.48 Calcium Chloride 0.43 0.2 M 4.056603774 Na-Cacodylate pH 7.5 100 mM Manganese Chloride 0.041 M 850 4.03 Lithium Sulfate 45.6 1.7 M 41.55528554 Na-Malonate pH 5.0 100 mM Trimethylamine HCl 0.002 M Monohydrate 851 8.04 Na—K-Phosphate pH 7.5 100 mM Trimethylamine HCl 0.031 M 852 1.52 Ferric Chloride 4 0.9 M 44.44444444 CAPSO pH 9.5 50 mM Strontium Chloride 0.002 M Hexahydrate 853 4.25 Zinc Sulfate 60 1.8 M 43.75 0 mM Hexahydrate 854 7.31 Ammonium 4.2588 4.3 M 29.6 0 mM ATP disodium salt 0.018 M Acetate 855 4.76 Ammonium 54.6 1.4 M 27.3 Mopso pH 6.5 100 mM Guanidine HCl 0.02 M Phosphate 856 7.74 Ammonium 0.84 0.8 M 12 Hepes pH 8.5 100 mM Sulfate 857 5.16 Lithium Sulfate 15.6 0.6 M 14.2162819 Na-Succinate pH 5.5 100 mM Xylitol 4.5% Monohydrate 858 9.49 Tri-Sodium Citrate 0.59 0.5 M 15.36458333 CAPSO pH 8.5 50 mM Dihydrate 859 1.46 Ferric Chloride 1 0.2 M 11.11111111 TRIS-Maleate pH 4.5 50 mM Taurine 0.048 M Hexahydrate 860 6.53 Ammonium 20 20.0% 10 Na-Succinate pH 6.5 100 mM Bromide 861 4.5 Ammonium 1.13 1.1 M 16.14285714 Na-Citrate pH 4.5 50 mM 1,4 Dioxane 3.1% Sulfate 862 6.98 Lithium Chloride 2.4 2.4 M 12 Na-Citrate pH 5.5 50 mM 863 6.52 Ammonium 2.77836 2.8 M 19.3 Na-Malonate pH 6.0 100 mM Cymal-3  0.2% Acetate 864 5.37 Na-Succinate pH 5.5 100 mM PEG 400 3% Triton X-100  0.2% 865 5.29 Sodium 0.1 0.1 M 8.6 CAPSO pH 8.5 50 mM Phosphate 866 4.36 Na-Succinate pH 4.5 100 mM D (+) Glucose 15% 867 6.29 Ammonium 1.05456 1.1 M 7.3 Na-Cacodylate pH 4.5 100 mM Ethylene Glycol 2.4% Acetate 868 5.35 Sodium Sulfate 0.095 0.1 M 7.421875 Na-Succinate pH 5.5 100 mM Trimethylamine HCl 0.067 M Maltoside  0.2% 869 6.48 Lithium Sulfate 42 1.5 M 38.2746051 Hepes pH 6.5 100 mM Ethylene Glycol 3.6% Monohydrate 870 3.46 Magnesium 0.85 0.8 M 18.75 TRIS-Maleate pH 4.5 50 mM Ethylene Glycol 1% Chloride 871 5.48 Calcium Acetate 26.8 0.4 M 17.86666667 Na-Cacodylate pH 5.5 100 mM Dihydrate 872 5.78 Calcium Chloride 0.56 0.2 M 5.283018868 TRIS-Maleate pH 6.5 50 mM Xylitol 1.5% 873 4.49 Na-Succinate pH 4.5 100 mM 2,2,2-Trifluoroethanol 14% 874 4.7 Ammonium 40 1.0 M 20 TRIS-HCl pH 7.5 100 mM Phosphate 875 5.74 Magnesium 0.45 0.5 M 9.9 Mopso pH 6.5 100 mM Sulfate 876 8.59 Sodium Formate 44.51 4.1 M 29.28571429 TRIS-HCl pH 8.5 100 mM Glycerol Anhydrous 3.5% 877 8.13 Potassium 1.73 0.5 M 15.01736111 Na—K-Phosphate pH 6.5 100 mM Phosphate 878 5.87 Ammonium 1.13 1.1 M 16.14285714 TRIS-Maleate pH 6.5 50 mM Ethyl Acetate 2.13% Sulfate 879 6.43 Tri-Sodium Citrate 0.852 0.7 M 22.1875 TRIS-Maleate pH 4.5 50 mM Phenol 0.091 M Dihydrate 880 6.92 Sodium Sulfate 0.138 0.2 M 10.78125 Na-Cacodylate pH 7.5 100 mM PEG 400 3.8% 881 8.59 Sodium Acetate 70.18 2.6 M 13 0 mM 1,4 Dioxane 1% 882 7.83 Ammonium 2.92 2.9 M 41.71428571 TRIS-HCl pH 7.5 100 mM Sulfate 883 6.69 Calcium Acetate 16.8 0.2 M 11.2 0 mM Manganese Chloride 0.006 M Glycerol   3% Dihydrate 884 1.42 Ferric Chloride 1.4 0.3 M 15.55555556 Hepes pH 7.5 100 mM Guanidine HCl 0.02 M Hexahydrate 885 3.96 Na-Malonate pH 6.0 100 mM ATP disodium salt 0.011 M 886 6.99 Potassium 30 1.1 M 35.27607362 Na—K-Phosphate pH 6.5 100 mM 1,4-Dithio-DL-Threitol 0.002 M Sodium Tartrate 887 5.26 Na-Acetate pH 5.5 50 mM PEG 400 10% 888 3.99 Na-Malonate pH 6.0 100 mM Guanidine HCl 0.2 M 889 6.29 Na-Succinate pH 6.5 100 mM ATP disodium salt 0.006 M 890 7.09 Calcium Acetate 40 0.5 M 26.66666667 Hepes pH 7.5 50 mM Dihydrate 891 2.01 Nickel Chloride 42.6 1.0% 12.5 Na-Citrate pH 5.5 50 mM Ethyl Acetate 1.14% Hexahydrate 892 7.36 Mopso pH 7.5 100 mM Glycine 0.298 M 893 2.18 Calcium Chloride 3.48 1.3 M 32.83018868 Na-Acetate pH 4.5 50 mM PEG 400 2% 894 7.75 Potassium 40 1.4 M 45 Bis-Tris Propane pH 7.5 100 mM Sodium Tartrate 895 1.54 Ferric Chloride 1.6 0.4 M 17.77777778 TRIS-HCl pH 8.5 100 mM 1,4 Butanediol 3% Glycerol   9% Hexahydrate 896 3.46 Zinc Sulfate 57 1.5 M 37.5 0 mM Yttrium Chloride 0.073 M Hexahydrate 897 5.11 Ammonium 9 2.0 M 9.838757396 Na-Citrate pH 5.5 50 mM Glycyl-Glycyl-Glycine 0.072 M Nitrate 898 4.96 Ammonium 24.55 1.3 M 26 TRIS-HCl pH 8.5 100 mM Benzamidine HCl 3.6% Glycerol  6.4% Citrate 899 9.26 Potassium 1.53 0.4 M 13.28125 Bis-Tris Propane pH 7.5 100 mM Trimethylamine HCl 0.006 M Phosphate 900 5.25 Ammonium 9.2 2.0 M 10.05739645 Na-Acetate pH 5.5 50 mM Yttrium Chloride 0.012 M Glycerol  3.6% Nitrate 901 6.59 TRIS-Maleate pH 6.5 50 mM Strontium Chloride 0.002 M 902 7.29 Potassium 58.2 1.2 M 38.33333333 Na-Cacodylate pH 7.5 100 mM Guanidine HCl 0.01 M Sodium Tartrate 903 7.01 Ammonium 1.9266 1.9 M 13.4 Na—K-Phosphate pH 6.5 100 mM Phenol 0.024 M Acetate 904 8.03 Hepes pH 8.5 100 mM Ethylene Glycol 25% 905 6.28 Mopso pH 7.5 100 mM Cupric Chloride 0.002 M 906 5.18 Potassium 39 2.1 M 25.7906843 Na-Citrate pH 5.5 50 mM Urea 0.06 M Triton X-100 0.88% Chloride 907 5.9 Sodium Sulfate 0.113 0.2 M 8.828125 0 mM sec-butanol 3.5% 908 5.37 Na—K-Phosphate pH 5.5 100 mM ATP disodium salt 0.01 M 909 4.23 Potassium 53.6 2.8 M 35.44565842 Na-Malonate pH 6.0 100 mM sec-butanol 3.9% Chloride 910 8.04 Lithium Chloride 4.15 4.2 M 20.75 Hepes pH 8.5 100 mM Trimethylamine HCl 0.075 M 911 4.46 Calcium Chloride 3.48 1.3 M 32.83018868 Bis-Tris Propane pH 8.5 100 mM 912 9.66 Calcium Acetate 5 0.1 M 3.333333333 CAPSO pH 9.5 50 mM Guanidine HCl 0.002 M Dihydrate 913 5.28 Na-Malonate pH 7.0 100 mM Taurine 0.033 M 914 5.19 Ammonium 41 1.0 M 20.5 Mopso pH 7.5 100 mM Xylitol 1% Phosphate 915 8.42 Ammonium 2.6 2.6 M 37.14285714 TRIS-HCl pH 8.5 100 mM PEG 400 4.7% Sulfate 916 4.63 Cadmium Sulfate 53.4 0.5 M 26.7 Bis-Tris Propane pH 9.5 100 mM Ethylene Glycol 4% Monohydrate 917 7.01 Potassium 20 0.7 M 23.51738241 Na-Acetate pH 5.5 50 mM MPD 3% Sodium Tartrate 918 8.43 Ammonium 2.92 2.9 M 41.71428571 Hepes pH 8.5 100 mM Guanidine HCl 0.024 M Sulfate 919 5.74 Calcium Chloride 0.6 0.2 M 5.660377358 Na-Cacodylate pH 6.5 100 mM 2,2,2-Trifluoroethanol 2.5% 920 9.16 Potassium 1.38 0.4 M 11.97916667 Na—K-Phosphate pH 7.5 100 mM Betaine Monohydrate 0.059 M Phosphate 921 8.12 TRIS-HCl pH 8.5 100 mM PEG 400 3.9% 922 7.49 Ammonium 4.49202 4.5 M 31.2 Na—K-Phosphate pH 8.5 100 mM 1,4 Butanediol 2.5% Acetate 923 4.64 Ammonium 37.8 0.9 M 18.9 Hepes pH 6.5 100 mM Xylitol 2.9% Phosphate 924 5.1 Ammonium 25.91 1.0 M 20 CAPSO pH 9.5 50 mM Dextran Sulfate 2.7% Citrate 925 6.19 Potassium 14.4 0.8 M 9.522714204 Mopso pH 6.5 100 mM Glycyl-Glycyl-Glycine 0.043 M Chloride 926 7.01 Ammonium 20 20.0% 10 TRIS-HCl pH 6.5 100 mM Bromide 927 6.34 Calcium Chloride 0.5 0.2 M 4.716981132 Bis-Tris Propane pH 6.5 100 mM Benzamidine HCl 0.5% 928 7.23 Tri-Sodium Citrate 0.38 0.3 M 9.895833333 Hepes pH 7.5 100 mM Dihydrate 929 7.19 Lithium Sulfate 46.6 1.7 M 42.46658566 CAPSO pH 8.5 50 mM Yttrium Chloride 0.002 M Monohydrate 930 5.52 Sodium Formate 29.11 3.5 M 25 Na-Acetate pH 5.5 50 mM Guanidine HCl 0.02 M 931 4.98 Cadmium Sulfate 38.8 0.4 M 19.4 0 mM 1,4 Butanediol 4.5% Monohydrate 932 7.99 Lithium Chloride 0.79 0.8 M 3.95 TRIS-HCl pH 8.5 100 mM D (+) Glucose 2.5% 933 4.24 Ammonium 40 1.0 M 20 TRIS-Maleate pH 4.5 50 mM Phosphate 934 8.69 Calcium Acetate 22 0.3 M 14.66666667 CAPSO pH 8.5 50 mM 2,2,2-Trifluoroethanol 2.1% Dihydrate 935 6.86 Sodium Sulfate 0.453 0.7 M 35.390625 TRIS-HCl pH 6.5 100 mM 936 6.97 Calcium Chloride 0.89 0.3 M 8.396226415 TRIS-HCl pH 7.5 100 mM 2,2,2-Trifluoroethanol 1% 937 6.94 Ammonium 10.4 10.4% 5.2 TRIS-Maleate pH 6.5 50 mM sec-butanol 3.8% Bromide 938 8.28 TRIS-HCl pH 7.5 100 mM Spermidine 0.02 M 939 6.38 Sodium 0.17 0.2 M 15 Na-Malonate pH 6.0 100 mM Phosphate 940 4.36 Lithium Chloride 8.81 8.5 M 42.5 TRIS-Maleate pH 6.5 50 mM Xylitol 1% Cymal-3  0.1% 941 5.63 Ammonium 10 2.2 M 10.93195266 0 mM Glycyl-Glycyl-Glycine 0.002 M Nitrate 942 8.59 Potassium 2.85 0.7 M 24.73958333 TRIS-Maleate pH 4.5 50 mM D (+) Glucose 2.5% Phosphate 943 7.09 Calcium Chloride 0.43 0.2 M 4.056603774 0 mM sec-butanol 1% 944 2.68 Nickel Chloride 44.4 0.8% 9.375 Bis-Tris Propane pH 9.5 100 mM Hexahydrate 945 8.55 Potassium 2 0.5 M 17.36111111 Bis-Tris Propane pH 9.5 100 mM Phosphate 946 8.52 Potassium 2.3 0.6 M 19.96527778 TRIS-HCl pH 6.5 100 mM Phosphate 947 4.27 Potassium 21.2 1.1 M 14.01955147 TRIS-Maleate pH 4.5 50 mM Glycerol Anhydrous 3.8% Chloride 948 4.36 Sodium 0.32 0.3 M 27.2 TRIS-Maleate pH 4.5 50 mM Benzamidine HCl 0.5% Phosphate 949 4.72 Ammonium 43.2 1.1 M 21.6 Na-Succinate pH 5.5 100 mM Taurine 0.013 M Phosphate 950 6.57 Ammonium 30 2.1 M 42 Na-Citrate pH 5.5 50 mM Yttrium Chloride 0.002 M Citrate 951 6.85 Sodium Acetate 20.71 2.5 M 12.5 TRIS-Maleate pH 6.5 50 mM 952 4.84 Cadmium Sulfate 46.6 0.6 M 31.06666667 TRIS-HCl pH 7.5 100 mM Glycine 0.1 M Maltoside 0.22% Monohydrate 953 9.57 Lithium Sulfate 27.8 1.0 M 25.33414338 CAPSO pH 9.5 50 mM Monohydrate 954 8.55 Potassium 1.26 0.3 M 10.9375 Bis-Tris Propane pH 9.5 100 mM MPD 4.6% Phosphate 955 6.94 Ammonium Acetate 1.10526 1.1 M 7.7 0 mM Taurine 0.025 M Triton X-100 0.36% 956 5.49 Ammonium 10.6 0.3 M 5.3 Na-Succinate pH 7.5 100 mM Benzamidine HCl 0.96% Phosphate 957 8.49 Potassium 40 2.1 M 26.4519839 Bis-Tris Propane pH 8.5 100 mM Chloride 958 8.38 Ammonium 1.07 1.1 M 15.28571429 Hepes pH 8.5 100 mM Xylitol 1.3% Sulfate 959 4.52 Sodium 0.295 0.3 M 25.4 0 mM Triton X-100 0.46% Phosphate 960 4.79 Ammonium 41.6 1.0 M 20.8 Mopso pH 6.5 100 mM PEG 400 1% Phosphate 961 5.48 Sodium Formate 27.36 2.0 M 14.28571429 Na-Succinate pH 5.5 100 mM Manganese Chloride 0.059 M 962 7.47 Sodium Chloride 3.51 3.5 M 36 TRIS-HCl pH 7.5 100 mM sec-butanol 3.8% 963 7.8 Sodium Formate 58.36 1.6 M 11.07142857 TRIS-HCl pH 7.5 100 mM Glycine 0.132 M 964 7.43 Sodium Formate 49.27 5.0 M 35.35714286 Mopso pH 7.5 100 mM Trimethylamine HCl 0.005 M 965 5.02 Ammonium 30 1.3 M 25 Hepes pH 8.5 100 mM Benzamidine HCl 3.44% Citrate 966 5.98 Cadmium Sulfate 50.8 0.7 M 33.86666667 Na-Malonate pH 6.0 100 mM Betaine Monohydrate 0.044 M Monohydrate 967 4.28 Ammonium 58.6 1.5 M 29.3 CAPSO pH 8.5 50 mM Ethylene Glycol 2.5% Cymal-3 0.34% Phosphate 968 8.09 Sodium Sulfate 0.26 0.4 M 20.3125 Hepes pH 8.5 100 mM 969 9.98 Tri-Sodium Citrate 0.702 0.6 M 18.28125 CAPSO pH 9.5 50 mM D (+) Glucose 1% Dihydrate 970 2.49 Magnesium 2.84 1.2 M 31.14035088 Na-Citrate pH 4.5 50 mM Dextran Sulfate 1.5% Chloride 971 6.49 Na-Succinate pH 6.5 100 mM 1,4-Dithio-DL-Threitol 0.056 M 972 5.02 Sodium 0.348 0.3 M 30 Hepes pH 6.5 100 mM Guanidine HCl 0.028 M Phosphate 973 5.42 Calcium Acetate 32 0.4 M 21.33333333 TRIS-Maleate pH 4.5 50 mM Manganese Chloride 0.041 M Dihydrate 974 5.97 Tri-Sodium Citrate 0.534 0.4 M 13.90625 Na-Succinate pH 4.5 100 mM Glycerol Anhydrous 1.4% Dihydrate 975 6.88 Potassium 10 0.5 M 6.612995975 Mopso pH 7.5 100 mM Manganese Chloride 0.01 M Chloride 976 7.04 Calcium Chloride 2.14 0.8 M 20.18867925 Mopso pH 7.5 100 mM 977 6.19 Ammonium 59.2 8.5 M 42.5 Na-Succinate pH 6.5 100 mM Xylitol 1% Nitrate 978 8.92 Tri-Sodium Citrate 0.96 0.8 M 25 Na-Malonate pH 7.0 100 mM Phenol 0.006 M Dihydrate 979 5.12 Ammonium 36 2.0 M 40 0 mM sec-butanol 3.4% Citrate 980 6.89 Sodium Chloride 2.44 2.4 M 25 Na-Cacodylate pH 7.5 100 mM 981 8.15 Ammonium 1.07 1.1 M 15.28571429 CAPSO pH 9.5 50 mM Betaine Monohydrate 0.074 M Sulfate 982 9.85 Potassium 3 0.8 M 26.04166667 Na-Succinate pH 6.5 100 mM Taurine 0.002 M Phosphate 983 3.8 Zinc Sulfate 28.4 1.5 M 38.66336634 Na-Succinate pH 4.5 100 mM Phenol 0.005 M Hexahydrate 984 8.29 Ammonium 1.71 1.7 M 24.42857143 Hepes pH 8.5 100 mM 1,4-Dithio-DL-Threitol 0.002 M Sulfate 985 7.33 Tri-Sodium Citrate 0.94 0.8 M 24.47916667 Bis-Tris Propane pH 9.5 100 mM Betaine Monohydrate 0.06 M Dihydrate 986 5.26 Ammonium 27.73 2.1 M 42.96197183 Mopso pH 7.5 100 mM Citrate 987 4.02 Magnesium 1.7 1.9 M 37.4 Na-Succinate pH 4.5 100 mM Ethylene Glycol 2.6% Sulfate 988 7.36 Potassium 29 1.0 M 34.1002045 0 mM D (+) Glucose 1% Sodium Tartrate 989 4.17 TRIS-Maleate pH 4.5 50 mM Strontium Chloride 0.03 M 990 5.43 Na—K-Phosphate pH 5.5 100 mM Benzamidine HCl 10% 991 6.91 Potassium 40 2.1 M 26.4519839 TRIS-HCl pH 6.5 100 mM Chloride 992 7.06 Potassium 36.2 1.3 M 42.56646217 Bis-Tris Propane pH 6.5 100 mM sec-butanol 1% Maltoside  0.1% Sodium Tartrate 993 4.53 Ammonium 19.2 19.2% 9.6 Na-Malonate pH 6.0 100 mM Urea 0.084 M Bromide 994 5.31 Calcium Chloride 1.47 0.6 M 13.86792453 Na-Cacodylate pH 6.5 100 mM MPD 4.7% 995 7.29 Ammonium 4.38048 4.4 M 30.4 0 mM Glycine 0.18 M Glucopyranoside  0.7% Acetate 996 6.13 Sodium Chloride 0.38 0.4 M 3.9 Na-Cacodylate pH 6.5 100 mM PEG 400 3.5% 997 7.95 CAPSO pH 8.5 50 mM Glycyl-Glycyl-Glycine 0.025 M 998 5 Sodium Formate 19.52 5.7 M 40.67852768 Na-Succinate pH 4.5 100 mM 1,4 Dioxane 1.1% 999 9.24 CAPSO pH 9.5 50 mM Dextran Sulfate 6% 1000 11.28 Na—K-Phosphate pH 8.5 100 mM Spermidine 0.04 M

REFERENCES Patents and Patent Publications:

-   U.S. Pat. No. 6,913,732 -   U.S. Pat. No. 6,039,804 -   U.S. Pat. No. 6,656,267 -   U.S. Pat. No. 7,214,540

Other References:

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1. A microplate, comprising a frame including a plurality of wells with defined side-by-side paired chambers of equal size, wherein the side-by-side paired chambers have a maximum volume of about 8 μl, and wherein the side-by-side paired chambers have a vapor channel providing vapor exchange between the side-by-side paired chambers.
 2. The microplate of claim 1, wherein the frame has a footprint that can be easily handled by a robotic handling system.
 3. The microplate of claim 1, wherein the side-by-side paired chambers have bottoms aligned in the same plane.
 4. The microplate of claim 1, wherein the side-by-side paired chambers have flat, conical, or concave bottoms.
 5. The microplate of claim 1, wherein the vapor channel has a predetermined depth and width to allow for a predetermined quantity of a first crystallization solution and a second crystallization solution to optimally equilibrate.
 6. The microplate of claim 1, wherein the vapor channel is formed by an opening in a wall between the side-by-side paired chambers and a membrane that is positioned over said plurality of wells.
 7. The microplate of claim 1, wherein each well is positioned on said frame such that a liquid handling system can automatically deposit a crystallization solution into one of the side-by-side paired chambers and can automatically deposit a protein solution into the other of the side-by-side paired chambers.
 8. The microplate of claim 1, wherein the microplate has 768 functional wells.
 9. The microplate of claim 8, wherein each well is positioned on said frame such that a liquid handling system can automatically deposit crystallization solution into one of the side-by-side paired chambers and can automatically deposit a protein solution into the other of the side-by-side paired chambers.
 10. A method of using a microplate comprising employing a liquid handling system to automatically deposit a crystallization solution into a first side-by-side paired chamber and to automatically deposit a protein solution into a second side-by-side paired chamber, wherein the side-by-side paired chambers each have a maximum volume of about 8 μl, wherein the crystallization solution and the protein solution interact via vapor diffusion; and wherein protein crystals are formed within the chamber containing the protein solution.
 11. The method of claim 10, wherein the crystallization solution is selected from the solutions shown in Table
 2. 12. The method of claim 10, wherein the amount of crystallization solution deposited is about 6 μl and the amount of protein solution deposited is about 1 μl.
 13. The method of claim 10, wherein the amount of crystallization solution deposited is in the range of about 4 μl to about 8 μl and the amount of protein solution deposited is in the range of greater than 0.5 μl to about 2 μl. 